Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples

The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research. However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohort...

Full description

Saved in:
Bibliographic Details
Published inCurrent molecular medicine Vol. 20; no. 10; p. 840
Main Authors Torok, Rebeka, Farkas, Anna, Guttman, Andras, Jarvas, Gabor
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.2020
Subjects
N-glycome
storage time
storage temperature
preanalytical processing
Blood sampling
capillary electrophoresis
Online AccessGet more information

Cover

More Information
Summary:The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research. However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies. In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection. Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance. Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results. Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures. Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.
ISSN:1875-5666
DOI:10.2174/1566524020666201230094722