Population-based p16 and HPV positivity rates in oropharyngeal cancer in Southeast Scotland

• Published in: Journal of clinical pathology Vol. 68; no. 10; pp. 849 - 852
• Main Authors: Wells, L A R, Junor, E J, Conn, B, Pattle, S, Cuschieri, K
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group LTD 01.10.2015
• Subjects: Alcohol; Biomarkers, Tumor - analysis; Carcinoma, Squamous Cell - chemistry; Carcinoma, Squamous Cell - epidemiology; Carcinoma, Squamous Cell - mortality; Carcinoma, Squamous Cell - pathology; Carcinoma, Squamous Cell - therapy; Carcinoma, Squamous Cell - virology; Cervical cancer; Chemotherapy; Cyclin-Dependent Kinase Inhibitor p16 - analysis; DNA, Viral - genetics; Female; Human papillomavirus; Human papillomavirus 16; Human Papillomavirus DNA Tests; Humans; Immunohistochemistry; Infections; Kaplan-Meier Estimate; Laboratories; Male; Medical prognosis; Middle Aged; Oropharyngeal Neoplasms - chemistry; Oropharyngeal Neoplasms - epidemiology; Oropharyngeal Neoplasms - mortality; Oropharyngeal Neoplasms - pathology; Oropharyngeal Neoplasms - therapy; Oropharyngeal Neoplasms - virology; Papillomaviridae; Papillomaviridae - genetics; Papillomavirus Infections - diagnosis; Papillomavirus Infections - epidemiology; Papillomavirus Infections - mortality; Papillomavirus Infections - therapy; Papillomavirus Infections - virology; Population; Predictive Value of Tests; Prevalence; Radiation therapy; Retrospective Studies; Risk Factors; Scotland - epidemiology; Time Factors; Treatment Outcome; Women; Scotland; HEAD AND NECK CANCER; PAPILLOMA VIRUSES; HPV; IMMUNOHISTOCHEMISTRY
• ISSN: 0021-9746; 1472-4146
• DOI: 10.1136/jclinpath-2015-202947

We assessed a population-based cohort of patients diagnosed with oropharyngeal squamous cell carcinoma in Southeast Scotland over 13 months. p16 and human papilloma virus (HPV) expression were determined, and correlated with stage, treatment, smoking and alcohol history, and disease outcomes. Retrospective analysis was performed on 60 patients. p16 immunohistochemistry and HPV genotyping were performed on formalin-fixed paraffin-embedded tissues. HPV infection (as defined by p16 positivity and/or HPV PCR positivity) was identified in 57% of samples, while dual positives were detected in 45% of cases. HPV16 was most prevalent of the HPV types and was associated with 90% of positive samples. Cause-specific 1-year and 2-year survivals were 82.5% and 78.2%, respectively. The p16-positive and HPV-positive groups demonstrated significantly increased cause-specific survival in comparison with their negative counterparts.