Trametes versicolor growth and laccase induction with by-products of pulp and paper industry
The cultivation of Trametes versicolor for laccase production and cell growth were strongly dependent on experimental conditions namely physical and chemical parameters as well as nutrient availability and inducer stimulation. Biomass growth was compared for a rich medium and for a defined medium in...
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Published in | Electronic Journal of Biotechnology Vol. 10; no. 3 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Chile
Universidad Católica de Valparaíso
15.07.2007
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Subjects | |
Online Access | Get full text |
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Summary: | The cultivation of Trametes versicolor for laccase production and cell
growth were strongly dependent on experimental conditions namely
physical and chemical parameters as well as nutrient availability and
inducer stimulation. Biomass growth was compared for a rich medium and
for a defined medium in two different temperatures. The best
temperature was 28°C and the maximum specific growth rates were
μmax = 0.083 h-1 for the rich medium and μmax = 0.043 h-1 for
the defined medium. It was clearly shown that laccase production is not
associated with cell growth, indicating that this ligninolytic enzyme
must be produced in the defined medium by a secondary metabolism. In
order to obtain laccase induction, addition of solid lignin,
lignosulphonates, veratryl alcohol, xylidine and ethanol was tested at
different concentrations. To optimise laccase activity, the combined
effect of inducer addition and simultaneously glucose suppression was
studied. The best result for laccase induction (1240 U/L) was obtained
with solid lignin, a by-product of pulp and paper industry and the
higher laccase activity attained (1583 U/L) was obtained with the
combined effect of xylidine addition and glucose suppression. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0717-3458 0717-3458 |
DOI: | 10.2225/vol10-issue3-fulltext-1 |