Low-protein diet in adult male rats has long-term effects on metabolism

• Published in: Journal of endocrinology Vol. 221; no. 2; pp. 285 - 295
• Main Authors: Malta, Ananda, de Oliveira, Júlio Cezar, Ribeiro, Tatiane Aparecida da Silva, Tófolo, Laize Peron, Barella, Luiz Felipe, Prates, Kelly Valério, Miranda, Rosiane Aparecida, Elmhiri, Ghada, Franco, Claudinéia Conationi da Silva, Agostinho, Aryane Rodrigues, Trombini, Amanda Bianchi, Pavanello, Audrei, Gravena, Clarice, Abdennebi-Najar, Latifa, Mathias, Paulo Cezar de Freitas
• Format: Journal Article
• Language: English
• Published: England Bioscientifica Ltd 01.05.2014; BioScientifica
• Subjects: Animals; Autonomic Nervous System - physiopathology; Birth Weight - drug effects; Birth Weight - physiology; Blood Glucose - metabolism; Cells, Cultured; Diet, Protein-Restricted - adverse effects; Dietary Proteins - metabolism; Insulin - metabolism; Islets of Langerhans - drug effects; Islets of Langerhans - innervation; Islets of Langerhans - metabolism; Life Sciences; Male; Rats; Rats, Wistar; Time Factors; protein restriction; autonomous nervous system; adulthood; pancreatic islets
• ISSN: 0022-0795; 1479-6805
• DOI: 10.1530/JOE-13-0473

Nutritional insults during developmental plasticity have been linked with metabolic diseases such as diabetes in adulthood. We aimed to investigate whether a low-protein (LP) diet at the beginning of adulthood is able to program metabolic disruptions in rats. While control rats ate a normal-protein (23%; NP group) diet, treated rats were fed a LP (4%; LP group) diet from 60 to 90 days of age, after which an NP diet was supplied until they were 150 days old. Plasma levels of glucose and insulin, autonomous nervous system (ANS), and pancreatic islet function were then evaluated. Compared with the NP group, LP rats exhibited unchanged body weight and reduced food intake throughout the period of protein restriction; however, after the switch to the NP diet, hyperphagia of 10% (P<0.05), and catch-up growth of 113% (P<0.0001) were found. The LP rats showed hyperglycemia, insulin resistance, and higher fat accretion than the NP rats. While the sympathetic tonus from LP rats reduced by 28%, the vagus tonus increased by 21% (P<0.05). Compared with the islets from NP rats, the glucose insulinotropic effect as well as cholinergic and adrenergic actions was unaltered in the islets from LP rats. Protein restriction at the beginning of adulthood induced unbalanced ANS activity and fat tissue accretion later in life, even without functional disturbances in the pancreatic islets.