Periodontal ligament stem cell isolation protocol: A systematic review

Despite the plethora of literature regarding isolation and characterization of periodontal ligament stem cells (PDLSCs), due to the existence of controversies in the results, in this comprehensive review, we aimed to summarize and compare the effect of isolation methods on PDLSC properties, includin...

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Published inCurrent stem cell research & therapy
Main Authors Rad, Maryam Rezai, Atarbasi-Moghadam, Fazele, Khodayari, Pouya, Sijanivandi, Soran
Format Journal Article
LanguageEnglish
Published United Arab Emirates 01.01.2022
Subjects
Enzymatic digestion
Explant
Periodontal ligament
Outgrowth
Periodontal ligament stem cell
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Summary:Despite the plethora of literature regarding isolation and characterization of periodontal ligament stem cells (PDLSCs), due to the existence of controversies in the results, in this comprehensive review, we aimed to summarize and compare the effect of isolation methods on PDLSC properties, including clonogenicity, viability/proliferation, markers expression, cell morphology, differentiation, and regeneration. Moreover, the outcomes of included studies, considering various parameters such as teeth developmental stages, donor age, periodontal ligament health status, and part of the teeth root from which PDLSCs were derived, have been systematically discussed. It has been shown that from included studies PDLSCs can be isolated from teeth from any developmental stages, any health status condition, and any donor age. Also, a non-enzymatic digestion method, named as an explant or outgrowth technique, is a suitable protocol for of PDLSCs isolation.
ISSN:2212-3946
DOI:10.2174/1574888X17666220128114825