Association of polymorphisms in the peroxisome proliferator-activated receptor γ gene and osteoarthritis of the knee

• Published in: Annals of the rheumatic diseases Vol. 65; no. 10; pp. 1394 - 1397
• Main Authors: Cheng, S, Afif, H, Martel-Pelletier, J, Benderdour, M, Pelletier, J-P, Hilal, G, Haraoui, P, Raynauld, J-P, Choquette, D, Fahmi, H
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and European League Against Rheumatism 01.10.2006; BMJ; BMJ Group
• Subjects: Age of Onset; Aged; Biological and medical sciences; Case-Control Studies; Concise Report; Diseases of the osteoarticular system; Female; Gene Frequency; Genetic Predisposition to Disease; Genotype; Haplotypes; Humans; Male; Medical sciences; Middle Aged; Miscellaneous. Osteoarticular involvement in other diseases; Osteoarthritis; Osteoarthritis, Knee - genetics; peroxisome proliferator-activated receptor; Polymorphism, Genetic; PPAR; PPAR gamma - genetics; Severity of Illness Index; Western Ontario and McMaster University Osteoarthritis Index; WOMAC Index; Knee; Knee osteoarthritis; Diseases of the osteoarticular system; Rheumatology; Arthropathy; Degenerative disease; PPAR-γ receptor; Osteoarthritis; Polymorphism
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/ard.2006.051904

Objectives: To study the association between two common polymorphisms in the peroxisome proliferator-activated receptor γ (PPARγ) gene and susceptibility to, and severity of, osteoarthritis in a French-Canadian population. Methods: Genomic DNA was obtained from 172 patients with osteoarthritis and 210 ethnically matched healthy controls. Genotyping for the polymorphisms in the PPARγ gene (Pro12Ala and C1431T) was carried out using polymerase chain reaction–restriction fragment length polymorphism. The standard Kellgren–Lawrence grading score and the French version of the Western Ontario and McMaster Universities Osteoarthritis Index were used to assess the radiological and functional severity of the disease. Estimated haplotypes were generated using the expectation maximisation algorithm. Genotype and allele frequencies were analysed using the χ2 test. Results: Genotype and allele frequencies for either polymorphism in the PPARγ gene did not differ significantly between patients with osteoarthritis and controls. Moreover, no significant differences were observed after stratification of patients according to age at disease onset, radiological or functional severity. Similarly, haplotype analysis of both polymorphisms in the PPARγ gene showed no association of any haplotype with susceptibility to, or severity of, osteoarthritis. Conclusion: These findings suggest that the examined polymorphisms in the PPARγ gene do not contribute to susceptibility to, or severity of, osteoarthritis in the French-Canadian population.