Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal

An improved and simplified method is described for the measurement of vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal. The extraction of serum in the presence of cyanide and the incorporation of B12-deficient serum into the intrinsic factor control has increased the accurac...

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Bibliographic Details
Published inJournal of clinical pathology Vol. 22; no. 2; pp. 205 - 211
Main Authors Raven, J. L., Robson, M. B., Walker, P. L., Barkhan, P.
Format Journal Article
LanguageEnglish
Published England BMJ Publishing Group Ltd and Association of Clinical Pathologists 01.03.1969
BMJ Publishing Group LTD
Subjects
Biological Assay
Charcoal
Cobalt Isotopes
Cyanides
Humans
Hydroxocobalamin - analysis
Intrinsic Factor
Lactobacillus
Methods
Vitamin B 12 - blood
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Summary:An improved and simplified method is described for the measurement of vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal. The extraction of serum in the presence of cyanide and the incorporation of B12-deficient serum into the intrinsic factor control has increased the accuracy of the method for both sera and crystalline B12 solutions. There are interesting differences between the results obtained for some sera by the isotope and L. leichmannii methods and the reasons for these differences are discussed.
Bibliography:href:jclinpath-22-205.pdf
PMID:5776552
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ark:/67375/NVC-PKNB8ZFP-Q
local:jclinpath;22/2/205
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.22.2.205