Long-term survival and aldosterone secretion pattern of aldosteronoma in culture

In an attempt to obtain an in vitro experimental model for aldosteronoma, primary culture was initiated with adenomas from 3 patients with primary aldosteronism. The cells grown in culture retained the morphology and functional properties characteristic of aldosteronoma cells well for periods of up...

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Published inActa endocrinologica (Copenhagen) Vol. 104; no. 4; pp. 495 - 501
Main Authors Okabe, T, Hidaka, H, Ohsawa, N, Tsushima, T
Format Journal Article
LanguageEnglish
Published Denmark 01.12.1983
Subjects
Adenoma - metabolism
Adenoma - pathology
Adrenal Gland Neoplasms - metabolism
Adrenal Gland Neoplasms - pathology
Adrenals
Adrenocorticotropic Hormone - pharmacology
Aldosterone - metabolism
Angiotensin I - pharmacology
Angiotensin II - pharmacology
Bucladesine - pharmacology
Cell Survival
Cells, Cultured
Humans
Hyperaldosteronism - metabolism
Hyperaldosteronism - pathology
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Summary:In an attempt to obtain an in vitro experimental model for aldosteronoma, primary culture was initiated with adenomas from 3 patients with primary aldosteronism. The cells grown in culture retained the morphology and functional properties characteristic of aldosteronoma cells well for periods of up to 200 days. The cells formed monolayer cell colonies and showed an epithelioid morphology with small nuclei containing prominent nucleoli. The cells possessed a clear, eosinophilic cytoplasm resembling that of aldosteronoma cells in vivo. The cultured cells continued to secrete large amounts of aldosterone throughout the culture period. The cells responded to angiotensin II and III by increased release of aldosterone into the culture medium. They also responded to Db-cAMP and ACTH by increased secretion of the hormone.
ISSN:0804-4643
0001-5598
1479-683X
DOI:10.1530/acta.0.1040495