Toxoplasma gondii Hsp70 as a danger signal in Toxoplasma gondii-infected mice

• Published in: Cell stress & chaperones Vol. 5; no. 4; pp. 328 - 335
• Main Authors: Mun, Hye-Seong, Aosai, Fumie, Norose, Kazumi, Chen, Mei, Hata, Hidekazu, Tagawa, Yoh-ichi, Iwakura, Yoichi, Byun, Dae-Seok, Yano, Akihiko
• Format: Journal Article
• Language: English
• Published: Netherlands Cell Stress Society International 01.10.2000
• Subjects: Animals; Antigens, Protozoan; Bradyzoites; Cysts; Heat-Shock Proteins - genetics; Heat-Shock Proteins - isolation & purification; HSP30 Heat-Shock Proteins; HSP70 Heat-Shock Proteins - isolation & purification; Immunity; Immunity, Innate; Infections; Interferon-gamma - genetics; Interferon-gamma - pharmacology; Macrophages, Peritoneal - metabolism; Membrane Proteins - genetics; Membrane Proteins - isolation & purification; Messenger RNA; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Nitric Oxide - metabolism; Original; Original s; Peritoneal macrophages; Protozoan Proteins - genetics; Protozoan Proteins - isolation & purification; Reverse transcriptase polymerase chain reaction; RNA, Messenger - isolation & purification; RNA, Protozoan - isolation & purification; T lymphocytes; Tachyzoites; Toxoplasmosis, Animal - mortality
• ISSN: 1355-8145; 1466-1268
• DOI: 10.1379/1466-1268(2000)005<0328:TGHAAD>2.0.CO;2

Toxoplasma gondii Hsp70, T gondii Hsp30/bag1, and surface antigen 1 messenger RNAs were shown to be useful in analyzing stage conversion of T gondii between bradyzoites and tachyzoites. The high-level expression of T gondii Hsp70 was correlated with mortality in interferon-γ knockout mice infected with T gondii. T gondii Hsp70 inhibited the induction of nitric oxide release by peritoneal macrophages of T gondii-infected mice. These findings identify T gondii Hsp70 as a danger signal during lethal, acute T gondii infection.