Curcumin-containing Silver Nanoparticles Prevent Carbon Tetrachloride- induced Hepatotoxicity in Mice

• Published in: Combinatorial chemistry & high throughput screening Vol. 24; no. 10; p. 1609
• Main Authors: Ebaid, Hossam, Habila, Mohamed, Hassan, Iftekhar, Al-Tamimi, Jameel, Omar, Mohamed S, Rady, Ahmed, Alhazza, Ibrahim M
• Format: Journal Article
• Language: English
• Published: United Arab Emirates 2021
• Subjects: Animals; Carbon Tetrachloride; Chemical and Drug Induced Liver Injury - drug therapy; Curcumin - chemistry; Curcumin - pharmacology; Liver - drug effects; Liver - metabolism; Male; Metal Nanoparticles - chemistry; Mice; Mice, Inbred C57BL; Silver - chemistry; Silver - pharmacology; silver nanoparticles; Carbon tetrachloride; hepatotoxicity; oxidative stress; curcumin; liver function
• ISSN: 1875-5402
• DOI: 10.2174/1386207323666201211100830

Hepatotoxicity remains an important clinical challenge. Hepatotoxicity observed in response to toxins and hazardous chemicals may be alleviated by delivery of the curcumin in silver nanoparticles (AgNPs-curcumin). In this study, we examined the impact of AgNPs-curcumin in a mouse model of carbon tetrachloride (CCl )-induced hepatic injury. Male C57BL/6 mice were divided into three groups (n=8 per group). Mice in group 1 were treated with vehicle control alone, while mice in Group 2 received a single intraperitoneal injection of 1 ml/kg CCl4 in liquid paraffin (1:1 v/v). Mice in group 3 were treated with 2.5 mg/kg AgNPs-curcumin twice per week for three weeks after the CCl challenge. Administration of CCL resulted in oxidative dysregulation, including significant reductions in reduced glutathione and concomitant elevations in the level of malondialdehyde (MDA). CCL challenge also resulted in elevated levels of serum aspartate transaminase (AST) and alanine transaminase (ALT); these findings were associated with the destruction of hepatic tissues. Treatment with AgNPs-curcumin prevented oxidative imbalance, hepatic dysfunction, and tissue destruction. A comet assay revealed that the CCl challenge resulted in significant DNA damage as documented by a 70% increase in nuclear DNA tail-length; treatment with AgNPscurcumin inhibited the CCL4-mediated increase in nuclear DNA tail-length by 34%. Administration of AgNPs-curcumin resulted in significant anti-oxidant activity in vivo. This agent has the potential to prevent hepatic tissue destruction and DNA damage that results from direct exposure to CCL .