House Flies in the Confined Cattle Environment Carry Non-O157 Shiga Toxin-Producing Escherichia coli

• Published in: Journal of medical entomology Vol. 54; no. 3; pp. 726 - 732
• Main Authors: Puri-Giri, R., Ghosh, A., Thomson, J. L., Zurek, L.
• Format: Journal Article
• Language: English
• Published: England Entomological Society of America 01.05.2017; Oxford University Press
• Subjects: Adulterants; Animal Husbandry; Animals; Bacteria; Beef; Beef cattle; Cattle; Cattle manure; Coliforms; Dairy farms; Dairying; E coli; Epidemiological Monitoring - veterinary; Escherichia coli; Factory farming; Farms; feedlot; Feedlots; Flies; house fly; Houseflies - microbiology; Immunomagnetic separation; Immunomagnetic Separation - veterinary; Insect Vectors - microbiology; Insects; Intensive farming; Multiplex Polymerase Chain Reaction - veterinary; Muscidae; non-O157 STEC; Pest outbreaks; Polymerase chain reaction; Reservoirs; Serogroup; Shiga toxin; Shiga-Toxigenic Escherichia coli - classification; Shiga-Toxigenic Escherichia coli - isolation & purification; Toxins; United States; VECTOR/PATHOGEN/HOST INTERACTION, TRANSMISSION; Virulence; feedlot; Shiga toxin; non-O157 STEC; house fly; Escherichia coli
• ISSN: 0022-2585; 1938-2928
• DOI: 10.1093/jme/tjw240

Cattle manure is one of the primary larval developmental habitats of house flies, Musca domestica (L.). Cattle serve as asymptomatic reservoirs of Shiga toxin-producing Escherichia coli (STEC), and bacteria are released into the environment in cattle feces. The USDA-FSIS declared seven STEC serogroups (O157, O26, O45, O103, O145, O121, and O111) as adulterants in beef products. In addition, the serogroup O104 was a culprit of a large outbreak in Germany in 2011. Our study aimed to assess the prevalence of seven non-O157 STEC (O26, O45, O145, O103, O121, O111, and O104) serogroups in adult house flies. Flies (n = 463) were collected from nine feedlots and three dairy farms in six states in the United States and individually processed. This involved a culturing approach with immunomagnetic separation followed by multiplex polymerase chain reactions for detection of individual serogroups and virulence traits. The concentration of bacteria on modified Possé agar ranged between 1.0 × 101 and 7.0 × 107 (mean: 1.5 ± 0.3 × 106) CFU/fly. Out of 463 house flies, 159 (34.3%) carried one or more of six E. coli serogroups of interest. However, STEC was found in 1.5% of house flies from feedlots only. These were E. coli O103 and O104 harboring stx1 and ehxA and E. coli O45 with stx1, eae, and ehxA. This is the first study reporting the isolation of non-O157 STEC in house flies from the confined cattle environment and indicating a potential role of this insect as a vector and reservoir of non-O157 STEC in confined beef cattle.