Effects of WT1 down-regulation on oocyte maturation and preimplantation embryo development in pigs

The Wilms' tumour 1 (WT1) gene originally identified as a tumour suppressor associated with WTs encodes a zinc finger-containing transcription factor that is expressed in multiple tissues and is an important regulator of cellular and organ growth, proliferation, development, migration and survi...

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Published inReproduction (Cambridge, England) Vol. 148; no. 4; pp. 377 - 387
Main Authors Gao, Fei, Guan, Jiyu, Liu, Limei, Zhang, Sheng, An, Peipei, Fan, Anran, Song, Guangqi, Zhang, Peng, Zhao, Tianchuang, Tang, Bo, Zhang, Xueming, Li, Ziyi
Format Journal Article
LanguageEnglish
Published England Bioscientifica Ltd 01.10.2014
Subjects
Animals
Apoptosis
Blastocyst - metabolism
Blastocyst - pathology
Cells, Cultured
Coculture Techniques
Down-Regulation
Embryo Culture Techniques
Female
Gene Expression Regulation, Developmental
In Vitro Oocyte Maturation Techniques
Male
Oocytes - metabolism
RNA Interference
RNA, Messenger - metabolism
Signal Transduction
Sperm Injections, Intracytoplasmic
Swine
Time Factors
Transfection
WT1 Proteins - genetics
WT1 Proteins - metabolism
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Summary:The Wilms' tumour 1 (WT1) gene originally identified as a tumour suppressor associated with WTs encodes a zinc finger-containing transcription factor that is expressed in multiple tissues and is an important regulator of cellular and organ growth, proliferation, development, migration and survival. However, there is a deficiency of data regarding the expression and function of WT1 during oocyte maturation and preimplantation embryonic development. Herein, we sought to define the expression characteristics and functions of WT1 during oocyte maturation and preimplantation embryonic development in pigs. We show that WT1 is expressed in porcine oocytes and at all preimplantation stages in embryos generated by ICSI. We then evaluated the effects of down-regulating WT1 expression at germinal vesicle and early ICSI stages using a recombinant plasmid (pGLV3-WT1-shRNA). Down-regulation of WT1 did not affect oocyte maturation but significantly decreased preimplantation embryonic development and increased apoptosis in blastocysts. These results indicate that WT1 plays important roles in the development of porcine preimplantation embryos.
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ISSN:1470-1626
1741-7899
DOI:10.1530/REP-14-0204