SUBCELLULAR DISTRIBUTION OF TESTOSTERONE METABOLITES AFTER PERFUSION OF ISOLATED LIVERS OF GUINEA PIG

ABSTRACT The isolated livers of four guinea pigs were perfused with 4-14C-testosterone. It was found, that 23.1 % of the initially added activity was retained in the liver. The subcellular distribution of this 14C-activity was investigated by differential centrifugation. After the extraction procedu...

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Bibliographic Details
Published inActa endocrinologica (Copenhagen) Vol. 62; no. 1; pp. 147 - 152
Main Authors Demisch, K, Birkelbach, P, Ammedick, U, Staib, W
Format Journal Article
LanguageEnglish
Published Denmark 01.09.1969
Subjects
Animals
Carbon Isotopes
Chromatography, Gas
Chromatography, Paper
Chromatography, Thin Layer
Guinea Pigs
Liver - metabolism
Testosterone - metabolism
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Summary:ABSTRACT The isolated livers of four guinea pigs were perfused with 4-14C-testosterone. It was found, that 23.1 % of the initially added activity was retained in the liver. The subcellular distribution of this 14C-activity was investigated by differential centrifugation. After the extraction procedure, the 14C-activity of the liver cell fractions was divided into A activity extractable by ether (free steroids), B activity remaining in the water phase (steroid-conjugates) and C activity associated with the filter residues (protein bound steroids). The ether extractable metabolites were separated by paper and thin layer chromatography and characterized by their gas chromatographic behaviour on a SE 30 and XE 60 column as free steroids, steroid-acetates and steroid-trimethylsilylethers. It was shown that there was no significant difference between the percentage distribution of free testosterone metabolites in the liver and perfusate. The steroid-conjugates had a three times higher concentration in the liver than in the perfusate. The subcellular distribution of free steroids showed significant differences. Of particular interest were the different ratios of testosterone to androstenedione in the subcellular fractions.
ISSN:0804-4643
0001-5598
1479-683X
DOI:10.1530/acta.0.0620147