BINDING OF CHOLECALCIFEROL METABOLITES TO RAT DUODENAL MUCOSA CYTOSOL

ABSTRACT In vitro binding of 25-hydroxycholecalciferol (25-(OH)D3) and 1α,25-dihydroxycholecalciferol (1,25-(OH)2D3) was studied in the duodenal mucosa cytosol of rachitic rats: both 25-(OH)D3 and 1,25-(OH)2D3 bind to a macromolecule (sedimentation coefficient 5.5 to 6 S in low or high ionic strengt...

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Bibliographic Details
Published inActa endocrinologica (Copenhagen) Vol. 84; no. 2; pp. 439 - 448
Main Authors Ulmann, A, Brami, M, Pezant, E, Garabedian, M, Funck-Bretano, J L
Format Journal Article
LanguageEnglish
Published Denmark 01.02.1977
Subjects
Animals
Binding Sites
Centrifugation, Density Gradient
Cholecalciferol - metabolism
Cytosol - metabolism
Dihydroxycholecalciferols - metabolism
Duodenum - metabolism
Hydroxycholecalciferols - metabolism
Intestinal Mucosa - metabolism
Male
Rats
Rickets - metabolism
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Summary:ABSTRACT In vitro binding of 25-hydroxycholecalciferol (25-(OH)D3) and 1α,25-dihydroxycholecalciferol (1,25-(OH)2D3) was studied in the duodenal mucosa cytosol of rachitic rats: both 25-(OH)D3 and 1,25-(OH)2D3 bind to a macromolecule (sedimentation coefficient 5.5 to 6 S in low or high ionic strength) with a high affinity (KD at 4°C = 1.2×10−9 m and 2×10−9 m, respectively). In addition, it is concluded from competition and chase experiments that 25-(OH)D3 binding sites differ from that for 1,25-(OH)2D3.
ISSN:0804-4643
0001-5598
1479-683X
DOI:10.1530/acta.0.0840439