Clinostomum complanatum and Clinostomum marginatum (Rudolphi, 1819) (Digenea: Clinostomidae) Are Separate Species Based on Differences in Ribosomal DNA

• Published in: The Journal of parasitology Vol. 90; no. 2; pp. 413 - 414
• Main Authors: Dzikowski, R, Levy, M. G, Poore, M. F, Flowers, J. R, Paperna, I
• Format: Journal Article
• Language: English
• Published: Lawrence, KS American Society of Parasitologists 01.04.2004
• Subjects: Animal genetics; animal taxonomy; Animals; Ardea herodias; Base Sequence; Biological and medical sciences; Biological taxonomies; Bird Diseases - parasitology; Birds; Clinostomidae; Cyprinidae - parasitology; DNA primers; DNA Primers - chemistry; DNA, Helminth - chemistry; DNA, Helminth - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Egretta; Egretta garzetta; Fish; Fish Diseases - parasitology; Fish Diseases - transmission; Foodborne illnesses; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Genetic Variation; Infections; internal transcribed spacers; Invertebrates; Molecular Sequence Data; molecular systematics; nucleotide sequences; Parasite hosts; Parasitology; Polymerase chain reaction; Polymerase Chain Reaction - veterinary; Polymorphism, Genetic; RESEARCH NOTES; Ribosomal DNA; sequence alignment; Sequence Alignment - veterinary; Species Specificity; taxonomy; Trematoda - classification; Trematoda - genetics; trematode infections; Trematode Infections - parasitology; Trematode Infections - veterinary; Helmintha; Plathelmintha; Parasite; Invertebrata; Trematoda; Ribosomal DNA
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/GE-159R

Infections by metacercariae of Clinostomum (Leidy, 1856) species adversely affect aquacultured fish and are potentially transmissible to humans. Molecular methodologies are efficient tools, which enable diagnosis of all life-history stages of trematodes in their diverse hosts. The small subunit of ribosomal DNA genes of adults of the Old World Clinostomum complanatum (Rudolphi, 1819) and the New World Clinostomum marginatum (Rudolphi, 1819), obtained from a little egret Egretta garzetta (Linnaeus, 1766) and the great blue heron Ardea herodias (Linnaeus, 1758), respectively, were amplified, sequenced, and aligned. The resulting alignment was used to develop a genetic assay to differentiate between these species.