Effects of bovine follicular fluid on the secretion of LH and FSH in inhibin-immunized seasonally anoestrous ewes

• Published in: Journal of endocrinology Vol. 128; no. 3; pp. 403 - 410
• Main Authors: Knight, P.G, Wrathall, J.H.M, Glencross, R.G, McLeod, B.J
• Format: Journal Article
• Language: English
• Published: Colchester BioScientifica 01.03.1991; Portland Press
• Subjects: Anestrus - metabolism; Animals; Biological and medical sciences; Cattle; cows; ewes; Female; Follicle Stimulating Hormone - blood; follicle-stimulating hormone; follicular fluid; Follicular Fluid - physiology; Fundamental and applied biological sciences. Psychology; gonadotropin-releasing hormone; Gonadotropin-Releasing Hormone - pharmacology; Gonadotropins, Pituitary - blood; hormone secretion; Hormones and neuropeptides. Regulation; Hypothalamus. Hypophysis. Epiphysis. Urophysis; immunization; Immunization Schedule; immunoneutralization; inhibin; Inhibins - immunology; Inhibins - physiology; luteinizing hormone; Luteinizing Hormone - blood; Seasons; Sheep - metabolism; Sheep - physiology; Vertebrates: endocrinology; Ovarian follicular fluid; Inhibin; Gonadotropin RH; Gonadotropin; Active immunization; Endocrine secretion; Hypothalamic hormone; Vertebrata; Mammalia; Adenohypophyseal hormone; Sheep; FSH; Artiodactyla; Hormone releasing factor; LH; Ungulata
• ISSN: 0022-0795; 1479-6805
• DOI: 10.1677/joe.0.1280403

ABSTRACT It has been shown previously that treatment of seasonally anoestrous ewes with steroid-free bovine follicular fluid (FF), a crude inhibin-containing preparation, leads to a decrease in plasma FSH level which is accompanied by a marked increase in pulsatile LH secretion. Since FF contains several factors (e.g. activin, follistatin, unidentified components) other than inhibin, which might act to modify gonadotrophin secretion, it was of interest to establish whether these concurrent effects of FF on FSH and LH secretion persisted in ewes which had been actively immunized against a synthetic peptide replica of the α subunit of bovine inhibin. In June 1989 (anoestrous period) groups of inhibin-immune and control ewes (n = 5 per group) received 6-hourly s.c. injections of either bovine serum (2 ml) or one of two doses of FF (0·5 ml or 2 ml) for 3 days. Blood was withdrawn at 6-h intervals for 6 days beginning 24 h before the first injection. On the final day of treatment, additional blood samples were withdrawn at 15-min intervals for 8 h to monitor pulsatile LH secretion. Ewes were then challenged with exogenous gonadotrophin-releasing hormone (GnRH; 2 μg i.v. bolus) to assess pituitary responsiveness. In control ewes, FF promoted a dose-dependent suppression of basal (maximum suppression 65%; P < 0·01) and post-GnRH (maximum suppression 72%; P < 0·01) levels of FSH in plasma. This was accompanied by an increase (P < 0·01) in LH pulse frequency from 1·40±0·24 (s.e.m.) to 3·20±0·37 pulses/8 h. In contrast, FF did not affect secretion of either FSH or LH in inhibin-immunized ewes. However, mean plasma LH levels in immunized ewes were significantly lower (43%; P < 0·02) than in control ewes, irrespective of treatment. These findings indicate that in the anoestrous ewe the ability of FF to suppress plasma FSH is due entirely to its content of inhibin, that FF-induced enhancement of pulsatile LH secretion is mediated by inhibin, rather than some additional component of FF, and that immunoneutralization of endogenous inhibin can reduce LH secretion. Journal of Endocrinology (1991) 128, 403–410