Serological characterisation and antimicrobial susceptibility of Actinobacillus pleuropneumoniae strains isolated from pigs in Spain

• Published in: Veterinary record Vol. 137; no. 3; p. 62
• Main Authors: Gutiérrez, C B, Rodríguez Barbosa, J I, Tascón, R I, Costa, L, Riera, P, Rodríguez Ferri, E F
• Format: Journal Article
• Language: English
• Published: England 15.07.1995
• Subjects: Actinobacillus Infections - epidemiology; Actinobacillus Infections - microbiology; Actinobacillus Infections - veterinary; Actinobacillus pleuropneumoniae - classification; Actinobacillus pleuropneumoniae - drug effects; Actinobacillus pleuropneumoniae - isolation & purification; Animals; Anti-Bacterial Agents - pharmacology; Disease Outbreaks - veterinary; Drug Resistance, Microbial; Lung - microbiology; Microbial Sensitivity Tests - veterinary; Pleuropneumonia - epidemiology; Pleuropneumonia - microbiology; Pleuropneumonia - veterinary; Serotyping - veterinary; Spain - epidemiology; Swine; Swine Diseases - epidemiology; Swine Diseases - microbiology; Spain
• ISSN: 0042-4900
• DOI: 10.1136/vr.137.3.62

Seventy-one isolates of Actinobacillus pleuropneumoniae isolated from the lungs of pigs in outbreaks of pleuropneumonia in Spain were serotyped by indirect haemagglutination. Serotype 4 (42.2 per cent), serotype 7 (22.5 per cent) and serotype 2 (12.8 per cent) were predominant, whereas serotypes 1, 3, 6, 8, 9, 12 and untypable isolates were present only in small numbers. Serotypes 1, 2, 4 and 7 originated mainly from cases of acute pleuropneumonia, whereas serotypes 3, 6, 8, 9 and 12 were associated with chronically infected herds. The susceptibility of the isolates to 20 antimicrobial agents was determined by agar disc diffusion. Most were susceptible to cefuroxime, cefaclor, cefazolin, kanamycin, tobramycin, gentamicin, oxolinic acid, ciprofloxacin, enoxacin, thiamphenicol, colistin and trimethoprim/sulphamethoxazole. Marked resistance was found with amoxicillin, ticarcillin, oxytetracycline, doxycycline and metronidazole. Rifampicin, fosfomycin and tiamulin were the agents most effective against the isolates tested.