MICROPROPAGATION OF ECLIPTA ALBA (L.) HASSK – AN IMPORTANT MEDICINAL PLANT

An efficient and reproducible protocol for mass propagation of Eclipta alba (L.) Hassk, an important medicinal plant, was standardized by culturing shoot tips and nodal segments taken from in vitro raised plants. Maximum shoot proliferation occurred when the explants were cultured on Murashige and S...

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Bibliographic Details
Published inIn vitro cellular & developmental biology. Plant Vol. 41; no. 5; pp. 658 - 661
Main Authors DHAKA, NEETI, KOTHARI, S. L.
Format Journal Article
LanguageEnglish
Published Heidelberg CABI Publishing 01.09.2005
Springer Nature B.V
Subjects
Asteraceae
benzyladenine
bhringraja
clonal propagation
culture media
Eclipta prostrata
gibberellic acid
Indian culture
indole butyric acid
mass multiplication
Medicinal plants
Micropropagation
nodal explant
Plant cells
Plant propagation
Plant tissues
Plantlets
Plants
Protocols/Methods
Rooting
shoot meristems
Shoot tip culture
stem nodes
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Summary:An efficient and reproducible protocol for mass propagation of Eclipta alba (L.) Hassk, an important medicinal plant, was standardized by culturing shoot tips and nodal segments taken from in vitro raised plants. Maximum shoot proliferation occurred when the explants were cultured on Murashige and Skoog (MS) medium supplemented with 1 mg l−1 benzylaminopurine (BAP). The shoot buds formed were further multiplied and maintained on medium containing BAP (0.5 mg l−1) and gibberellic acid (0.5 mg l−1). Rooting was best achieved on MS medium supplemented with 1 mg−1 indole-3-butyric acid. Rooted plantlets attained maturity and flowered normally in the field.
ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2005684