Excessive proliferation and apoptosis of parathyroid cells contribute to primary hyperparathyroidism in rabbit model

To explore the molecular pathogenesis of primary hyperparathyroidism (PHPT), we investigated the proliferation and apoptosis of parathyroid cells in a rabbit model of diet-induced PHPT. A total of 120 adult Chinese rabbits were randomly divided into normal diet (Ca:P, 1:0.7) group (control group) or...

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Published inJournal of investigative medicine Vol. 70; no. 6; pp. 1392 - 1398
Main Authors Bi, Jing-tao, Bai, Rong-jie, Zhan, Hui-li, Qian, Zhan-hua, Gong, Li-hua, Liu, Ya-qi, Zheng, Zhi-xue, Cai, Xuan
Format Journal Article
LanguageEnglish
Published Los Angeles, CA BMJ Publishing Group Ltd 01.08.2022
SAGE Publications
Sage Publications Ltd
Subjects
Animals
Antibodies
Apoptosis
Cell cycle
Cell growth
cell proliferation
disease models, Animal
Endocrine system
Hyperplasia
Lymphoma
Original research
Pathogenesis
Pathology
Phosphorus
Protein expression
Proteins
Rabbits
Thyroid gland
Tumors
Beijing China
United States > US
China
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Summary:To explore the molecular pathogenesis of primary hyperparathyroidism (PHPT), we investigated the proliferation and apoptosis of parathyroid cells in a rabbit model of diet-induced PHPT. A total of 120 adult Chinese rabbits were randomly divided into normal diet (Ca:P, 1:0.7) group (control group) or a high-phosphate diet (Ca:P, 1:7) group (experimental group). The thyroid and parathyroid complexes were harvested for 1-month interval from month 1 to month 6. The expression of proliferation markers, including proliferating cell nuclear antigen (PCNA) and cyclin-D1, and B cell lymphoma-2 (Bcl-2), were evaluated by immunohistochemistry in thyroid and parathyroid tissues. Apoptosis was quantified by DNA-fragment terminal labeling. Our results demonstrated that parathyroid cells in the experimental group started proliferating from the end of the 2nd month, the expression of PCNA, Bcl-2, and cyclin-D1 were significantly higher in the PHPT group than those of the control group (p<0.05). Furthermore, the apoptosis index (AI) was positively correlated with the glandular cell count and expression of PCNA in the 6th month in the PHPT group. Overall, our results suggested that excessive proliferation and apoptosis of parathyroid cells may contribute to the pathogenesis of PHPT through PCNA-related, Bcl-2-related, and cyclin-D1-related pathways.
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ISSN:1081-5589
1708-8267
DOI:10.1136/jim-2021-002184