Haploid androgenetic development of bovine embryos reveals imbalanced WNT signaling and impaired cell fate differentiation

• Published in: Biology of reproduction Vol. 109; no. 6; pp. 821 - 838
• Main Authors: Aguila, Luis, Nociti, Ricardo P., Sampaio, Rafael V., Therrien, Jacinthe, Meirelles, Flavio V., Felmer, Ricardo N., Smith, Lawrence C.
• Format: Journal Article
• Language: English
• Published: United States Society for the Study of Reproduction 11.12.2023; Oxford University Press
• Subjects: Animals; blastocyst; Blastocyst - metabolism; Cattle; Cell Differentiation - genetics; Embryonic Development - genetics; Gene Expression Regulation, Developmental; Haploidy; Humans; imprinted genes; RESEARCH ARTICLE; uniparental embryos; Wnt Signaling Pathway - genetics; imprinted genes; uniparental embryos; blastocyst
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1093/biolre/ioad124

Haploid embryos have contributed significantly to our understanding of the role of parental genomes in development and can be applied to important biotechnology for human and animal species. However, development to the blastocyst stage is severely hindered in bovine haploid androgenetic embryos (hAE). To further our understanding of such developmental arrest, we performed a comprehensive comparison of the transcriptomic profile of morula-stage embryos, which were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) of transcripts associated with differentiation in haploid and biparental embryos. Among numerous disturbances, results showed that pluripotency pathways, especially the wingless-related integration site (WNT) signaling, were particularly unbalanced in hAE. Moreover, transcript levels of KLF4, NANOG, POU5F1, SOX2, CDX2, CTNNBL1, AXIN2, and GSK3B were noticeably altered in hAE, suggesting disturbance of pluripotency and canonical WNT pathways. To evaluate the role of WNT on hAE competence, we exposed early Day-5 morula stage embryos to the GSK3B inhibitor CHIR99021. Although no alterations were observed in pluripotency and WNT-related transcripts, exposure to CHIR99021 improved their ability to reach the blastocysts stage, confirming the importance of the WNT pathway in the developmental outcome of bovine hAE. Summary Sentence The importance of the wingless-related integration site pathway on bovine haploid androgenetic development by transcriptomic analysis and identifies pluripotency markers associated with cell fate determination during early development. Graphical Abstract