THE SPECIFICITY OF THE MOUSE OVARIAN AUGMENTATION ASSAY FOR FOLLICLE STIMULATING HORMONE

ABSTRACT The specificity of the mouse ovarian augmentation assay for gonadotrophins has been examined. Variations of the augmenting dose of HCG from 12.5 to 100 IU per mouse had no significant influence on the assay characteristics. However, the addition of ovine luteinising hormone to standard gona...

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Bibliographic Details
Published inActa endocrinologica (Copenhagen) Vol. 56; no. 2; pp. 359 - 368
Main Authors Martin, F I, Peyton, L
Format Journal Article
LanguageEnglish
Published Denmark 01.10.1967
Subjects
Animals
Biological Assay
Chorionic Gonadotropin - pharmacology
Chorionic Gonadotropin - standards
Female
Follicle Stimulating Hormone - pharmacology
Follicle Stimulating Hormone - standards
Mice
Organ Size
Ovary - drug effects
Ovary - physiology
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Summary:ABSTRACT The specificity of the mouse ovarian augmentation assay for gonadotrophins has been examined. Variations of the augmenting dose of HCG from 12.5 to 100 IU per mouse had no significant influence on the assay characteristics. However, the addition of ovine luteinising hormone to standard gonadotrophins produced a depression of the expected response in all standards tested. Similarly, the assay of mixtures of standard gonadotrophins did not produce the additive response expected from the simultaneous assay of the standards alone. These effects were less marked when pure human pituitary preparations were assayed. It is concluded that although this assay is sensitive and precise it cannot be regarded as specific for follicle stimulating hormone.
ISSN:0804-4643
0001-5598
1479-683X
DOI:10.1530/acta.0.0560359