IN VITRO GROWTH KINETICS AND THERMOSTABILITY OF THE GOLDEN SHINER VIRUS
In vitro replication of the golden shiner virus (GSV) occurred at 20 C to 30 C in fathead minnow cells; GSV did not replicate at 15 C or 35 C. Optimal replication temperature was approximately 30 C, where the latent period was 8 hr and viral yield was 113 infectious units per cell. Maximum cytopathi...
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Published in | Journal of wildlife diseases Vol. 18; no. 4; pp. 441 - 446 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
Wildlife Disease Association
01.10.1982
Wildlife Dis Assoc |
Subjects | |
Online Access | Get full text |
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Summary: | In vitro replication of the golden shiner virus (GSV) occurred at 20 C to 30 C in fathead minnow cells; GSV did not replicate at 15 C or 35 C. Optimal replication temperature was approximately 30 C, where the latent period was 8 hr and viral yield was 113 infectious units per cell. Maximum cytopathic effect coincided with maximum released viral titer. Suitable storage temperatures for GSV were −70 C and 4 C. Viral infectivity was rapidly lost at temperatures above 20 C and at normal freezer temperature (−15 C). |
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Bibliography: | L73 8250044 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0090-3558 1943-3700 |
DOI: | 10.7589/0090-3558-18.4.441 |