IN VITRO GROWTH KINETICS AND THERMOSTABILITY OF THE GOLDEN SHINER VIRUS

In vitro replication of the golden shiner virus (GSV) occurred at 20 C to 30 C in fathead minnow cells; GSV did not replicate at 15 C or 35 C. Optimal replication temperature was approximately 30 C, where the latent period was 8 hr and viral yield was 113 infectious units per cell. Maximum cytopathi...

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Bibliographic Details
Published inJournal of wildlife diseases Vol. 18; no. 4; pp. 441 - 446
Main Authors SCHWEDLER, T.E, PLUMB, J.A
Format Journal Article
LanguageEnglish
Published United States Wildlife Disease Association 01.10.1982
Wildlife Dis Assoc
Subjects
Animals
Cell Line
Cyprinidae
Cytopathogenic Effect, Viral
Epithelium
Fishes - microbiology
RNA Viruses - growth & development
RNA Viruses - pathogenicity
Temperature
VIROLOGY
Virulence
Virus Replication
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Summary:In vitro replication of the golden shiner virus (GSV) occurred at 20 C to 30 C in fathead minnow cells; GSV did not replicate at 15 C or 35 C. Optimal replication temperature was approximately 30 C, where the latent period was 8 hr and viral yield was 113 infectious units per cell. Maximum cytopathic effect coincided with maximum released viral titer. Suitable storage temperatures for GSV were −70 C and 4 C. Viral infectivity was rapidly lost at temperatures above 20 C and at normal freezer temperature (−15 C).
Bibliography:L73
8250044
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0090-3558
1943-3700
DOI:10.7589/0090-3558-18.4.441