The utility of IS6110 sequence based polymerase chain reaction in comparison to conventional methods in the diagnosis of extra-pulmonary tuberculosis
IS6110 sequence based polymerase chain reaction (PCR) was compared with conventional bacteriological techniques in the laboratory diagnosis of extra-pulmonary tuberculosis (EPTB). One hundred and ninety one non-repeated clinical samples of EPTB and 17 samples from non-tuberculous cases as controls w...
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Published in | Indian journal of medical microbiology Vol. 26; no. 4; pp. 352 - 355 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
India
Medknow Publications on behalf of Indian Association of Medical Microbiology
01.10.2008
Elsevier B.V |
Subjects | |
Online Access | Get full text |
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Summary: | IS6110 sequence based polymerase chain reaction (PCR) was compared with
conventional bacteriological techniques in the laboratory diagnosis of
extra-pulmonary tuberculosis (EPTB). One hundred and ninety one
non-repeated clinical samples of EPTB and 17 samples from
non-tuberculous cases as controls were included. All the samples were
processed for Ziehl-Neelsen staining for acid fast bacilli (AFB) and
143 samples were processed by culture for M. tuberculosis . All the
samples were processed for PCR amplification with primers targeting 123
bp fragment of insertion element IS6110 of M. tuberculosis complex. Of
the total 191 samples processed, 34 (18%) were positive by smear for
AFB. Culture for AFB was positive in 31(22%) samples among the 143
samples processed. Either smear or culture for AFB was found positive
in 51(27%) samples. Of the total 191 samples processed 120 (63%) were
positive by PCR. In 140 samples, wherein both the conventional
techniques were found negative, 74 (53%) samples were positive by PCR
alone. Among 51 samples positive by conventional techniques, 46 (90%)
were found positive by PCR. PCR assay targeting IS6110 is useful in
establishing the diagnosis of EPTB, where there is strong clinical
suspicion, especially when the conventional techniques are negative. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0255-0857 |
DOI: | 10.1016/S0255-0857(21)01813-2 |