Functional expression of the oxytocin receptor in Xenopus laevis oocytes primed with mRNA from bovine endometrium

ABSTRACT Synthesis of the uterine receptor for the hypothalamic hormone oxytocin has been induced in oocytes from Xenopus laevis previously primed with bovine endometrium mRNA. The injected oocytes responded to oxytocin by showing dose-dependent oscillations in membrane currents as recorded by the v...

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Bibliographic Details
Published inJournal of molecular endocrinology Vol. 1; no. 1; pp. 77 - 81
Main Authors Morley, S D, Meyerhof, W, Schwarz, J, Richter, D
Format Journal Article
LanguageEnglish
Published England BioScientifica 01.07.1988
Subjects
Animals
Cattle
Cell Membrane - physiology
Cell Membrane - ultrastructure
Dose-Response Relationship, Drug
Endometrium - analysis
Female
Membrane Potentials - drug effects
Microinjections
Oocytes - cytology
Oocytes - metabolism
Oocytes - ultrastructure
Receptors, Angiotensin - metabolism
Receptors, Angiotensin - physiology
Receptors, Oxytocin
RNA, Messenger - administration & dosage
RNA, Messenger - analysis
RNA, Messenger - pharmacology
Xenopus laevis - physiology
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Summary:ABSTRACT Synthesis of the uterine receptor for the hypothalamic hormone oxytocin has been induced in oocytes from Xenopus laevis previously primed with bovine endometrium mRNA. The injected oocytes responded to oxytocin by showing dose-dependent oscillations in membrane currents as recorded by the voltage-clamp method. The response was specific in that it was not elicited by several other peptides tested. The oxytocin-induced membrane changes were suppressed when oocytes were pretreated with an oxytocin receptor antagonist.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0952-5041
1479-6813
DOI:10.1677/jme.0.0010077