SAT0182 Relationship Between HLA-A Or -B LOCI and Behcet’S Disease in Japan. B51 is Related to Pathogenesis and A26 is Related to Ocular Lesions

• Published in: Annals of the rheumatic diseases Vol. 72; no. Suppl 3; p. A643
• Main Authors: Kobashigawa, T., Nanke, Y., Kawamoto, M., Yago, T., Yamanaka, H., Kotake, S.
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and European League Against Rheumatism 01.06.2013; BMJ Publishing Group LTD
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/annrheumdis-2013-eular.1908

Background Behçet’s disease (BD) was reported to be associated with 6 human leukocyte antigen (HLA) loci. (1-7) In our 1st report on the relationships between BD and the HLA-A or B locus, we demonstrated that HLA-B27 and B51 were associated with the onset of BD, and HLA-A26 was associated with ocular lesions of BD; however, analyses of the HLA locus lacked integrity. (9) Objectives To investigate the relationships between the development or symptoms of BD and HLA-A/B loci. Methods Three hundred and nine out-patients with BD visited our hospital from January 2009 to December 2011. Out of the 179 out-patients diagnosed with definite or probable BD by Japanese and international criteria, and who had visited since September 2006, after our 1st HLA survey for our BD out-patients, we enrolled 69 patients with BD: 18 males, 51 females; mean age (SD, median, 25th and 75th percentile), 41.0 (13.3, 41, 31, and 53) years old, enough to survey the associations of HLA loci in our BD out-patients and the pathogenesis of BD. Each doctor of the patients obtained informed consent, and HLA loci were analyzed with PCR-rSSO methods. The HLA data from the HLA laboratory (8) were used as data for the healthy control group. We statistically analyzed the relationships between BD lesions and each HLA-A/B locus. Results The characteristics of BD out-patients were as follows: 21(30.4%) ocular lesion; 67 (97.1%) oral aphthous ulcers, 54 (78.3%) genital lesions, 65 (94.2%) skin lesions, 38 (55.1%) cases of arthritis, 17 (24.6%) intestinal lesions, 5 (7.3%) CNS, 3 (4.4%) vascular lesions, 2 (11.1%) cases of epidydimitis out of 18 males, and 1 (1.5%) positive pathergy test. Moreover, 17.4% were the complete type and 82.6% were the incompletetype, in Japanese BD criteria. The HLA-B51 (p=0.0395, OR: 1.66, 95% CI: 1.03-2.68) was strongly associated with development of BD based on our case-control study. The locus HLA-A26 (p=0.015, OR: 4.09, 95% CI: 1.35-12.38) was significantly correlated with ocular lesions of BD after analyzing the relationships between 24 loci and the 10 lesions of BD in our patients. Conclusions An HLA locus, B51, was related to the development of BD. A significant correlation between HLA loci and BD lesions was detected between the HLA-A26 locus and ocular lesions. References Akpolat T et al., Eur J Med, 1992. Itoh Y et al., Tissue Antigens, 2006. Mizuki N et al., Tissue Antigens, 1997. Kaya TI et al., Clin Exp ermatol, 2002. Gul A et al., Genes and Immunity, 2002. Ohno S et al., Lancet, 1973. Paul M et al., Tissue Antigens, 2001. The HLA laboratory, http://www.hla.or.jp. Kobashigawa et al. EULAR2008 (abstract). Disclosure of Interest None Declared