Cytotoxicity of Nanoliposomal Cisplatin Coated with Synthesized Methoxypolyethylene Glycol Propionaldehyde in Human Ovarian Cancer Cell Line A2780CP
Purpose: To evaluate the cytotoxicity of pegylated nanoliposomal cisplatin on human ovarian cancer cell line A2780CP. Methods: Synthesized methoxypolyethylene glycol (mPEG) propionaldehyde was characterized by 1Hnuclear magnetic resonance (1H-NMR) and Fourier transform infrared spectroscopy (FTIR) a...
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Published in | Tropical journal of pharmaceutical research Vol. 15; no. 3; p. 563 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Nigeria
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
01.03.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Purpose: To evaluate the cytotoxicity of pegylated nanoliposomal
cisplatin on human ovarian cancer cell line A2780CP. Methods:
Synthesized methoxypolyethylene glycol (mPEG) propionaldehyde was
characterized by 1Hnuclear magnetic resonance (1H-NMR) and Fourier
transform infrared spectroscopy (FTIR) and used as coating agent for
the preparation of liposomal nanodrug formulation by reverse phase
evaporation method. The characteristics of the nanoparticles were
evaluated by dynamic light scattering (DLS) and scanning electron
microscopy (SEM). Encapsulation efficiency was determined
spectrometrically at 214.42 nm by inductively coupled plasma
spectroscopy (ICP-OES). The cytotoxicity of both pegylated
nanoliposomal and free cisplatin were evaluated by 3- [4,
5-dimethyl-2-thiazolyl] -2, 5- diphenyltetrazolium bromide (MTT) assay
and expressed as half-maximal inhibitory concentration (IC50). Results:
The mean diameter and zeta potential of drug-loaded liposomal particles
and empty nanoliposomes were 125 ± 2.9 nm and -16.6 mV, 108 ±
2.2 nm and -27.2 mV, respectively, while the cytotoxicity (IC50) of
free cisplatin and nanodrug formulation were 93.6 ± 3.1 μg/mL
and 67.8 ± 2.3 μg/mL, respectively. In vitro toxicological
results indicate that the formulation exhibited approximately 1.4-fold
cytotoxicity compared with the free drug. Drug encapsulation efficiency
of the nanoliposomes was approximately 98 ± 1 %. Conclusion: The
findings show that the cytotoxicity of pegylated nanoliposomal
cisplatin is higher than that of free cisplatin in human ovarian cancer
cell line A2780CP. In vivo studies are, however, required to ascertain
its therapeutic potentials. |
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ISSN: | 1596-5996 1596-5996 1596-9827 |
DOI: | 10.4314/tjpr.v15i3.18 |