Identification of Mycobacterium kansasii and a Mycobacterium sp. in Salmonids from the Missouri River, Montana

We collected fish from the Missouri River, Montana, to investigate a 1984 finding of Mycobacterium chelonae (reclassified as M. salmoniphilum) from Mountain Whitefish (Prosopium williamsoni). Brown Trout (Salmo trutta), Mountain Whitefish, and Rainbow Trout (Oncorhynchus mykiss) were sampled from th...

Full description

Saved in:
Bibliographic Details
Published inNorthwestern naturalist (Olympia, Wash.) Vol. 97; no. 2; pp. 98 - 104
Main Authors Terrazas, Marc M, Bradway, Daniel S, Staigmiller, Kenneth D, Wipf, Matthew M, Snekvik, Kevin R
Format Journal Article
LanguageEnglish
Published Winthrop Society for Northwestern Vertebrate Biology 22.09.2016
THE SOCIETY FOR NORTHWESTERN VERTEBRATE BIOLOGY
Subjects
Animal diseases
Bacteria
Colleges & universities
Deoxyribonucleic acid
DNA
Fish
Freshwater fishes
Fungal diseases
Fungal infections
Health aspects
Histology
Identification
Infections
Laboratories
Montana
Mountain Whitefish
Mycobacteria
Mycobacterium
Oncorhynchus mykiss
Pathogens
Population
Prosopium williamsoni
Rainbow Trout
REGULAR ARTICLE
Trout
Tuberculosis
Whitefish
United States
Missouri River
Montana
California
Online AccessGet full text

Cover

More Information
Summary:We collected fish from the Missouri River, Montana, to investigate a 1984 finding of Mycobacterium chelonae (reclassified as M. salmoniphilum) from Mountain Whitefish (Prosopium williamsoni). Brown Trout (Salmo trutta), Mountain Whitefish, and Rainbow Trout (Oncorhynchus mykiss) were sampled from the Missouri River in 2009–2010, to examine for the presence of tissue nodules and granulomatous inflammation indicative of mycobacterial infection. Mycobacterium kansasii and a Mycobacterium sp. were detected by tissue smears and histology and identified by direct sequencing in tissue samples of 1.7% of Mountain Whitefish and 3.3% of Rainbow Trout. Genotypes were identified from 16S-23S rRNA. This study confirmed the continued presence of Mycobacterium infection in Mountain Whitefish from the Missouri River, with an updated diagnosis and identified infection in Rainbow Trout. This is the 1st documented case of Mycobacterium kansasii in wild fish and the freshwater documentation of a species similar to Mycobacterium sp. YM12, which was sequenced from a marine fish.
ISSN:1051-1733
1938-5315
DOI:10.1898/NWN15-22.1