Bypassing the midgut results in development of Plasmodium berghei oocysts in a refractory strain of Anopheles gambiae (Diptera: Culicidae)

The L35 strain of Anopheles gambiae Giles was genetically selected for its ability to melanize and kill malaria parasites. A wide range of Plasmodium species are subject to this response when orally ingested, including the rodent malaria, P. berghei. However, when we directly injected P. berghei int...

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Bibliographic Details
Published inJournal of medical entomology Vol. 42; no. 4; pp. 712 - 715
Main Authors Paskewitz, S.M, Shi, L
Format Journal Article
LanguageEnglish
Published England 01.07.2005
Subjects
Animals
Anopheles - anatomy & histology
Anopheles - parasitology
Anopheles gambiae
biological development
immune response
infection
injection
insect vectors
Insect Vectors - parasitology
malaria
midgut
oocysts
Oocysts - growth & development
ookinetes
oral infection
parasite melanization
Plasmodium berghei
Plasmodium berghei - growth & development
strain differences
vector competence
vector refractoriness
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Summary:The L35 strain of Anopheles gambiae Giles was genetically selected for its ability to melanize and kill malaria parasites. A wide range of Plasmodium species are subject to this response when orally ingested, including the rodent malaria, P. berghei. However, when we directly injected P. berghei into the hemocoel, we found that parasites developed normally to the oocyst stage. This work suggests that the parasite melanization response depends on the interaction of the ookinetes and the midgut. This result is surprising because it contrasts with a genetically validated model system, where injection of CM-Sephadex beads directly into the hemocoel results in bead melanization.
ISSN:0022-2585
1938-2928
DOI:10.1603/0022-2585%282005%29042%5B0712%3ABTMRID%5D2.0.CO%3B2