Bovine luteal cell production in vitro of prostaglandin E2, oxytocin and progesterone in response to pregnancy-specific protein B and prostaglandin F2α

• Published in: Journal of reproduction & fertility Vol. 107; no. 1; p. 131
• Main Authors: Del Vecchio, R P, Sutherland, W D, Sasser, R G
• Format: Journal Article
• Language: English
• Published: England Society for Reproduction and Fertility 01.05.1996
• Subjects: Animals; Aspartic Acid Endopeptidases - pharmacology; Cattle; Cells, Cultured; Corpus Luteum - cytology; Corpus Luteum - drug effects; Corpus Luteum - metabolism; Dinoprost - pharmacology; Dinoprostone - metabolism; Female; Luteal Phase; Oxytocin - metabolism; Pregnancy Proteins - pharmacology; Progesterone - metabolism
• ISSN: 1470-1626; 0022-4251; 1741-7899
• DOI: 10.1530/jrf.0.1070131

A study was conducted to determine the effects of pregnancy-specific protein B (PSPB) and prostaglandin F 2α (PGF 2α ) on bovine luteal cell progesterone, prostaglandin E 2 (PGE 2 ) and oxytocin production in vitro . Corpora lutea were enucleated from multiparous cows with normal oestrous cycles during the mid-luteal (days 10–12; n = 5) or late-luteal (days 17–18; n = 5) stage. Mixed large and small cells (1.5 × 10 5 cells per well) were incubated in 500 μl modified Ham's F-12 medium. Cells were incubated for 18 h before treatments were added. Cells were treated with PSPB (0, 2.5, 5.0 μg) and PGF 2α (0, 100, 200 ng) in a 3 × 3 factorial arrangement. After treatments were added, media samples were collected at 6 and 12 h. During the 18 h pretreatment incubation, progesterone, PGE 2 and oxytocin production was similar between the prospective treatment groups. Progesterone production was greater ( P < 0.001) by mid-stage than by late-stage cells. In addition, progesterone decreased ( P < 0.001) as incubation time increased. Progesterone production was not affected by PGF 2α , but PSPB increased ( P < 0.02) progesterone at the 5.0 μg dose. Late-stage luteal cells produced more ( P < 0.001) PGE 2 than did mid-stage cells; PGE 2 production decreased ( P < 0.001) with increased incubation time. Luteal PGE 2 production increased in response to PSPB treatment ( P < 0.01) and PGF 2α treatment ( P < 0.001). Luteal oxytocin production was greater ( P < 0.01) by mid-stage compared with late-stage cells. Oxytocin production decreased ( P < 0.001) with incubation time in mid-stage cells, but in late-stage cells oxytocin production was similar over time. Neither PSPB nor PGF 2α had an effect on oxytocin. These results indicate that PSPB does not affect luteal oxytocin, but does increase progesterone and PGE 2 production. In addition, PGF 2α increases luteal PGE 2 , but does not affect progesterone or oxytocin production. These data do not show an interaction between PSPB and PGF 2α in regulating bovine luteal cell endocrine function.