Preparation of a partially desialylated human chorionic gonadotrophin (hCG) and its use for induction of ovulation after ovarian stimulation with human menopausal gonadotrophin

A method for the large scale preparation of partially desialylated human chorionic gonadotrophin suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The prepa...

Full description

Saved in:
Bibliographic Details
Published inActa endocrinologica (Copenhagen) Vol. 95; no. 2; pp. 232 - 236
Main Authors Crosignani, P G, Donini, P, Lombroso, G C, Donini, S, Caccamo, A, Trojsi, L
Format Journal Article
LanguageEnglish
Published Denmark 01.10.1980
Subjects
Adult
Amenorrhea - drug therapy
Animals
Asialoglycoproteins
Chorionic Gonadotropin - isolation & purification
Chorionic Gonadotropin - pharmacology
Estradiol - blood
Female
Humans
Menotropins - pharmacology
Neuraminidase
Ovaries
Ovary - drug effects
Ovulation - drug effects
Pregnancy
Progesterone - blood
Rats
Sepharose
Sialic Acids - analysis
Ultrafiltration
Online AccessGet full text

Cover

More Information
Summary:A method for the large scale preparation of partially desialylated human chorionic gonadotrophin suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The preparation showed to be active in vitro (OAAD and SVW tests) and its half-life was found to be 13 min in the rat and 75 min in human beings. This desialo hCG proved to be effective in inducing ovulation in amenorrhoeic women. Among 39 induced cycles 31 ovulations and 5 pregnancies occurred.
ISSN:0804-4643
0001-5598
1479-683X
DOI:10.1530/acta.0.0950232