Functional repertoire convergence of distantly related eukaryotic plankton lineages revealed by genome-resolved metagenomics

• Published in: bioRxiv
• Main Authors: Delmont, Tom O, Morgan, Gaia, Hinsinger, Damien D, Fremont, Paul, Vanni, Chiara, Antonio Fernandez Guerra, Eren, A Murat, Kourlaiev, Artem, Leo D’agata, Clayssen, Quentin, Villar, Emilie, Labadie, Karine, Cruaud, Corinne, Poulain, Julie, Da Silva, Corinne, Wessner, Marc, Noel, Benjamin, Jean-Marc Aury, Tara Oceans Coordinators, De Vargas, Colomban, Bowler, Chris, Karsenti, Eric, Pelletier, Eric, Wincker, Patrick, Jaillon, Olivier
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 23.01.2021; Cold Spring Harbor Laboratory
• Edition: 1.2
• Subjects: Biogeochemical cycles; Classification; Convergence; Culture collections; Genomes; Metagenomics; Microbiology; Oceans; Plankton; ecology; Oceans; functions; metagenomics; genomics; anvi’o; single-cell genomics; phylogeny; open ocean; plankton; evolution; Marine eukaryotes
• ISSN: 2692-8205; 2692-8205
• DOI: 10.1101/2020.10.15.341214

Abstract Marine planktonic eukaryotes play a critical role in global biogeochemical cycles and climate. However, their poor representation in culture collections limits our understanding of the evolutionary history and genomic underpinnings of planktonic ecosystems. Here, we used 280 billion Tara Oceans metagenomic reads from polar, temperate, and tropical sunlit oceans to reconstruct and manually curate more than 700 abundant and widespread eukaryotic environmental genomes ranging from 10 Mbp to 1.3 Gbp. This genomic resource covers a wide range of poorly characterized eukaryotic lineages that complement long-standing contributions from culture collections while better representing plankton in the upper layer of the oceans. We performed the first comprehensive genome-wide functional classification of abundant unicellular eukaryotic plankton, revealing four major groups connecting distantly related lineages. Neither trophic modes of plankton nor its vertical evolutionary history could explain the functional repertoire convergence of major eukaryotic lineages that coexisted within oceanic currents for millions of years. Cover Navigating on the map of plankton genomics with Tara Oceans and anvi’o: a comprehensive genome-resolved metagenomic survey dedicated to eukaryotic plankton. Figure1 Figure1 * Download figure * Open in new tab Competing Interest Statement The authors have declared no competing interest. Footnotes * The functional repertoire classification of unicellular eukaryotes is now performed in both the known and unknown coding space. This additional analysis. This allowed us to identify a functional repertoire convergence of distantly related eukaryotic plankton lineages in both the known and unknown coding sequence space, the latter representing a substantial amount of biologically relevant gene diversity. * https://www.genoscope.cns.fr/tara/