AB0614 A Comphehensive Analysis of 12 Disease-Specific Autoantibodies in Greek Patients with Systemic Sclerosis

• Published in: Annals of the rheumatic diseases Vol. 75; no. Suppl 2; p. 1114
• Main Authors: Liaskos, C., Marou, E., Simopoulou, T., Barmakoudi, M., Scheper, T., Meyer, W., Katsiari, C.G., Bogdanos, D., Sakkas, L.I.
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group LTD 01.06.2016
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/annrheumdis-2016-eular.4355

BackgroundPatients with systemic sclerosis (SSc) are characterized by ANAs against various autoantigens, none of which is 100% sensitive for the disease. The need for profiling testing based on a combination of the most important SSc-related autoantigens has led to the development of commercial SSc-profile assays.ObjectivesTo determine the diagnostic and clinical relevance of systemic sclerosis (SSc)-related autoantibodies tested by a line SSc-profile assay in a well defined cohort of Caucasian SSc patients, all from Central Greece.Methods98 consecutive patients with SSc (80 female, 18 male, mean age 57,9±14,9 y) including 60 patients with diffuse cutaneous SSc (dcSSc) and 38 with limited cutaneous SSc (lcSSc) were analyzed by a profile SSc line assay (Euroimmun) for autoantibodies against 12 autoantigens: Scl-70, Centromere (A, B), RNA polymerase III (subunits 11 & 155), fibrillarin, NOR90, Th/To, PM/Scl 100, PM/Scl75, Ku and PDGFR.ResultsANA by indirect immunofluorescence was present in 98 (98%) patients with SSc. Overall, 81 (82.6%) patients had abs against at least one of the 12 autoantigens. Anti-Scl-70 abs were present in 49 (49.4%) SSc patients, anti-centromere A in 22 (22.5%), anti-centromere B in 22 (22.5%, all anti-centromere A positive) anti-RNA polymerase III RP155) in 18 (18.3%) anti-RNA polymerase III RP11 in 14 (14.3%), anti-fibrillarin in 3 (3.1%), anti-Ku in 5 (5.1%), anti-NOR90 in 7 (7.1%), anti-PM/Scl100 in 4 (4.1%), anti-PM/Scl75 in 7 (7.1%), anti-Th/To in 4 (4.1%) and anti-PGDFR in 1 (1%) SSc patients. Overall, the most frequent anti-Scl-70, anti-centromere or anti-RNA polymerase III abs were present in 81 (81%) SSc patients while 6 (6.1%) lacked those antibodies and were positive for at least one of the other 9. Anti-Scl-70 were associated with diffuse SSc and ACA with limited SSc (p<0.001 for both). Positivity of Scl-70 was also correlated with the presence of lung fibrosis (p=0.004) and tended to be associated with the presence of digital ulcers (p=0.073). Anti-centromere abs were less prevalent in SSc patients with lung fibrosis (p=0.004). Anti-PM/Scl75 abs tended to be associated with the presence of digital ulcers (p=0.055). Anti-NOR90 abs were more frequent in male SSc patients (p=0.019).ConclusionsAnti-Scl70, anti-centromere and anti-RNA polymerase are by far the most dominant autoantibodies in SSc but other disease-related autoantibodies can also be present in SSc patients lacking those three autoantibodies. The presence of anti-Scl-70 abs identifies patients with lung fibrosis and along with anti-PM/Scl75 tend to be associated with the presence of digital ulcers.Disclosure of InterestC. Liaskos: None declared, E. Marou: None declared, T. Simopoulou: None declared, M. Barmakoudi: None declared, T. Scheper Employee of: Euroimmun AG, W. Meyer Employee of: Euroimmun AG, C. Katsiari: None declared, D. Bogdanos: None declared, L. Sakkas: None declared