P145 An intra-familial transmission of HIV-1 CRF02_AG was reconstructed by molecular epidemiology

• Published in: Sexually transmitted infections Vol. 95; no. Suppl 1; p. A123
• Main Authors: Huang, Chun, Xin, Ruolei, Bai, Lishi, Lu, Hongyan, Sun, Lijun, Liu, An
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group LTD 01.07.2019
• Subjects: Disease transmission; Drug resistance; Epidemiology; HIV; Human immunodeficiency virus; Infections
• ISSN: 1368-4973; 1472-3263
• DOI: 10.1136/sextrans-2019-sti.312

BackgroundMother to child transmission (MTCT) plays an important role in children HIV infection, and in China, about 0.1% to 0.4% individuals were infected through MTCT. Nucleic aicd amplification testing was powerful tool to assess the effect of prevention of MTCT, mainly by qualification NAT. Here, we described an intrafamiliar transmission event by molecular epidemiology.MethodsA pregnant woman with seroconversion during pregnant healthcare was followed up by epidemiology survey, together with her husband and newborn. Viral RNA was extracted for the amplification of HIV-1 gag gene and pol gene fragments with One-step RT-PCR. The positive PCR products were subject to DNA sequencing. The sequences of gag gene and pol gene were codon-based aligned and analyzed with MEGA 6.0 to construct neighbor-joining tree, respectively. The genotypic drug resistances were interpretated by Stanford University HIV drug resistance database.ResultsThe proband was the pregnant woman, with seroconversion of anti-HIV-antibody screening by Wantai HIV-1/2 Ab kit during pregnant healthcare. Afterward, her husband and son at age of eight months were diagnosed as HIV-1 infection by HIV-1 antibody screening and RT-PCR, respectively, although her son was RT-PCR negative one day after birth. Neighbor-joining (NJ) tree indicated that the three individuals were infected by HIV-1 CRF02_AG, forming a close subcluster with high genetic homogeneity (bootstrap value, 95%). The sequences from the husband were closer to the root or the ancestor of the tree, and the topology structure indicated the transmission timing and evolution relationship, from father to mother and child, as shown by seroepidemiology.ConclusionAn intra-familial transmission of HIV-1 CRF02_AG was reconstructed by molecular epidemiology and the possible transmission relationship was elucidated. Contagious diseases screening should be reinforced during healthcare before marriage or pregnancy-deliver. Prevention of mother-to-child transmission should be timely and efficiently administered upon diagnosis of infection in pregnant women, to avoid the secondary intra-familial transmission and improve the population quality at birth.DisclosureNo significant relationships.