IDDF2020-ABS-0199 Histopathological and immunohistochemical characteristics of hepatocellular carcinoma with portal vein thrombosis

• Published in: Gut Vol. 69; no. Suppl 2; p. A89
• Main Authors: Hien, Le Thi Thu, Tuan, Le Quoc, Duat, Nguyen Quang
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group LTD 01.11.2020
• Subjects: Angiogenesis; Biopsy; Cell differentiation; Hepatocellular carcinoma; Immunohistochemistry; Liver cancer; Portal vein; Thrombosis; Tumors; Ultrasound
• ISSN: 0017-5749; 1468-3288
• DOI: 10.1136/gutjnl-2020-IDDF.168

BackgroundDetection of portal vein thrombosis (PVT), especially benign or neoplastic PVT in new diagnosed hepatocellular carcinoma (HCC) has key meaning for prognosis as well as making the choice of treatment methods.ObjectivesTo analysis of histopathological and immunohistochemical characteristics of hepatocellular carcinoma with portal vein thrombosis(1)To the analysis of histopathological and immunohistochemical characteristics of hepatocellular carcinoma with portal vein thrombosis(2)To analyse the histopathological and immunohistochemical characteristics of hepatocellular carcinoma with portal vein thrombosisMethodsWe conducted a prospective study of 50 patients with HCC have PVT at Clinic 103 Cam Khe from June 2017 to August 2019. The PVT specimens were collected by biopsy through the skin according to ultrasound guidance. Immunohistochemistry test: the dyeing was performed by BondMax automatic dyeing machine (Leica Biosystems - Australia).ResultsThe location of HCC tumors associated with the location of PVT (p<0.01). Thromboses in the trunk of the portal vein of 56%, thromboses in the branches of the portal vein of 44%. The degree of thrombosis significantly related to the size of HCC tumor (p<0.01). All of the PVT in patients with HCC were malignant thromboses with moderate differentiation of 58%, poor differentiation of 36%, high differentiation of 6%. Neoangiogenesis in the thromboses by using immunohistochemistry: low level of 6%, moderate level of 46% and a high level of 48%. The neoangiogenesis in the thromboses significantly related to cancer cell differentiation (p<0.01).ConclusionsThe more angiogenesis was, the lower the grate of cell differentiation.