IDDF2021-ABS-0211 Co-occurrence of gut microbiota dysbiosis and bile acid metabolism alteration is associated with psychological disorders in crohn’s disease

• Published in: Gut Vol. 70; no. Suppl 2; pp. A144 - A145
• Main Authors: Gao, Xuefeng, Feng, Lijuan, Zhou, Nan, Li, Zichun, Fu, Dongni, Guo, Ying, Liu, Xiaowei
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group LTD 01.09.2021
• Subjects: Acids; Bile; Bile acids; Biodiversity; Central nervous system; Crohn's disease; Deoxycholic acid; Dysbacteriosis; Enterobacteriaceae; Fecal microflora; Feces; Intestinal microflora; Liquid chromatography; Mass spectroscopy; Metabolism; Metabolites; Microbiota; Mucosa; rRNA 16S; Taurodeoxycholic acid
• ISSN: 0017-5749; 1468-3288
• DOI: 10.1136/gutjnl-2021-IDDF.176

BackgroundMetabolites of the gut microbiota interviewing with signals derived from the host immune, endocrine, and neuronal systems intertwining may influence the development of mucosal injury and inflammation and impacts also distal central nervous system, underlying the psychophysiological vulnerability of Crohn’s disease (CD) patients. This study aims to elucidate the relationships between bile acid metabolism, gut microbiota, and psychological comorbidity in CD.MethodsA total 39 CD patients and 14 healthy controls were enrolled in this study. The psychological status of the participants was accessed by using Zung Self-rating Depression Scale (SDS) and Self-rating Anxiety Scale (SAS). Bile acids in the feces and serum samples were quantified by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). 16S rRNA gene sequencing was used to profile the fecal microbiota.ResultsA significant reduction in the secondary bile acids lithocholic acid (LCA) and deoxycholic acid (DCA) were found in both the feces and serum samples in CD patients. 7- hyodeoxycholic (DHCA) was found to be higher in the serum of CD patients with psychological disorder (SDS≥50 SAS≥45), and showed a positive correlation with SDS. In feces, SAS correlated positively with the levels of taurodeoxycholic acid (TDCA), taurolithocholic acid (TLCA), and tauro beta-muricholic acid (TbMCA), and SDS inversely correlated with HDCA and 12-DHCA. The fecal microbiota biodiversity was reduced in patients with CD, and further declined in those with psychological disorder. The microbiota composition of CD was featured by enrichment of Enterobacteriaceae and a loss of Faecalibacterium prausnitzii. Changes in the gut bacterial communities correlated directly with the psychological status of CD patients (such as Ruminococcus gnavus), and/or indirectly via affecting the SAS/SDS-associated bile acids (such as Enterobacteriaceae and Lachnospiraceae).ConclusionsChanges in the gut microbiota composition may cooperate with alterations in the bile acid metabolism that involved in the development of psychological disorders in CD.