Uncharted biosynthetic potential of the ocean microbiome

Microbes are phylogenetically and metabolically diverse. Yet capturing this diversity, assigning functions to host organisms and exploring the biosynthetic potential in natural environments remains challenging. We reconstructed >25,000 draft genomes, including from >2,500 uncharacterized speci...

Full description

Saved in:
Bibliographic Details
Published inbioRxiv
Main Authors Paoli, Lucas, Ruscheweyh, Hans-Joachim, neris, Clarissa C, Satria Kautsar, Clayssen, Quentin, Salazar, Guillem, Milanese, Alessio, Gehrig, Daniel, Larralde, Martin, Carroll, Laura, Sanchez, Pablo, Zayed, Ahmed A, Cronin, Dylan R, Acinas, Silvia G, Bork, Peer, Bowler, Chris, Delmont, Tom O, Sullivan, Matthew B, Wincker, Patick, Zeller, Georg, Robinson, Serina L, Piel, Joern, Sunagawa, Shinichi
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 24.03.2021
Cold Spring Harbor Laboratory
Edition1.1
Subjects
Bioactive compounds
Candidatus Eudoremicrobiaceae
Gene clusters
Genomes
Microbiology
Microbiomes
Natural products
Phylogeny
Transcription
meta-omics
natural products
Eudoremicrobium
Eudoremicrobiaceae
Amphithoemicrobium
genome-resolved microbiomics
biosynthetic potential
Autonoemicrobium
Ocean microbiome
Online AccessGet full text

Cover

More Information
Summary:Microbes are phylogenetically and metabolically diverse. Yet capturing this diversity, assigning functions to host organisms and exploring the biosynthetic potential in natural environments remains challenging. We reconstructed >25,000 draft genomes, including from >2,500 uncharacterized species, from globally-distributed ocean microbial communities, and combined them with ~10,000 genomes from cultivated and single cells. Mining this resource revealed ~40,000 putative biosynthetic gene clusters (BGCs), many from unknown phylogenetic groups. Among these, we discovered Candidatus Eudoremicrobiaceae as one of the most biosynthetically diverse microbes detected to date. Discrete transcriptional states structuring natural populations were associated with a potentially niche-partitioning role for BGC products. Together with the characterization of the first Eudoremicrobiaceae natural product, this study demonstrates how microbiomics enables prospecting for candidate bioactive compounds in underexplored microbes and environments. Competing Interest Statement The authors have declared no competing interest.
Bibliography:SourceType-Working Papers-1
ObjectType-Working Paper/Pre-Print-1
content type line 50
Competing Interest Statement: The authors have declared no competing interest.
ISSN:2692-8205
2692-8205
DOI:10.1101/2021.03.24.436479