Light microscopy of proteins in their ultrastructural context

Resolving the distribution of specific proteins at the nanoscale in the structural context of the cell is a major challenge in fluorescence microscopy. Here we present a new concept that decrowds the intracellular space through 13 to 21-fold physical expansion while simultaneously retaining the prot...

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Bibliographic Details
Published inbioRxiv
Main Authors Ons M'saad, Bewersdorf, Joerg
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 14.03.2020
Cold Spring Harbor Laboratory
Edition1.1
Subjects
Cell Biology
Fluorescence microscopy
Light microscopy
Microscopy
Proteins
Proteomes
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Summary:Resolving the distribution of specific proteins at the nanoscale in the structural context of the cell is a major challenge in fluorescence microscopy. Here we present a new concept that decrowds the intracellular space through 13 to 21-fold physical expansion while simultaneously retaining the proteins. This combination makes labeling of the proteome efficient enough that local protein densities are revealed and the cellular nanoarchitecture can be visualized by standard light microscopy.
Bibliography:SourceType-Working Papers-1
ObjectType-Working Paper/Pre-Print-1
content type line 50
ISSN:2692-8205
2692-8205
DOI:10.1101/2020.03.13.989756