Unclearing Microscopy

The spatial resolution and contrast sensitivity of the human eye is limited, restricting our ability to directly see subcellular structures. We report a new principle for unaided eye cellular visualization in a method we call Unclearing Microscopy. By expanding cells and tissue >8,000 volumetrica...

Full description

Saved in:
Bibliographic Details
Published inbioRxiv
Main Authors Ons M'saad, Shribak, Michael, Bewersdorf, Joerg
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 02.12.2022
Cold Spring Harbor Laboratory
Edition1.1
Subjects
Bioengineering
Electron microscopy
Light microscopy
Optics
Patent applications
Spatial discrimination
Online AccessGet full text

Cover

More Information
Summary:The spatial resolution and contrast sensitivity of the human eye is limited, restricting our ability to directly see subcellular structures. We report a new principle for unaided eye cellular visualization in a method we call Unclearing Microscopy. By expanding cells and tissue >8,000 volumetrically and opaquing their bulk with light-scattering molecules of sufficient density, cell microstructure can now be discerned with a contrast visible to the unaided eye. We further inspect uncleared samples with transmitted light microscopy modalities and prove that 3D ultrastructural features, previously accessible only with super-resolution fluorescence or electron microscopy methods, can now be visualized with simple magnification optics alone.Competing Interest StatementJ.B. has financial interests in Bruker Corp. and Hamamatsu Photonics. O.M. and J.B. filed patent applications with the U.S. Patent and Trademark Office covering the presented method. O.M. and J.B. are co-founders of panluminate Inc. which is developing related products.
Bibliography:SourceType-Working Papers-1
ObjectType-Working Paper/Pre-Print-1
content type line 50
Competing Interest Statement: J.B. has financial interests in Bruker Corp. and Hamamatsu Photonics. O.M. and J.B. filed patent applications with the U.S. Patent and Trademark Office covering the presented method. O.M. and J.B. are co-founders of panluminate Inc. which is developing related products.
ISSN:2692-8205
2692-8205
DOI:10.1101/2022.11.29.518361