Observation of the Glycines in Elastin Using 13C and 15N Solid-State NMR Spectroscopy and Isotopic Labeling

We report the solid-state 13C and 15N NMR of insoluble elastin which has been synthesized in vitro with isotopically enriched glycine. Most of the glycines reside in a domain with good cross-polarization (CP) efficiencies, although surprisingly, a portion resides in an environment that is not detect...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 124; no. 24; pp. 6832 - 6833
Main Authors Perry, Ashlee, Stypa, Michael P, Foster, Judith A, Kumashiro, Kristin K
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 19.06.2002
Subjects
Applied sciences
Exact sciences and technology
Natural polymers
Physicochemistry of polymers
Proteins
Geometrical structure
Solid state
Nitrogen 15
Isotope labelling
Molecular structure
Elastin
Biopolymer
NMR spectrometry
Carbon 13
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Summary:We report the solid-state 13C and 15N NMR of insoluble elastin which has been synthesized in vitro with isotopically enriched glycine. Most of the glycines reside in a domain with good cross-polarization (CP) efficiencies, although surprisingly, a portion resides in an environment that is not detectable using CP. Our data indicate that much of the 13C population resides in regions of significant conformational flexibility. To support these conclusions, we present 13C and 15N cross-polarization with magic-angle-spinning (CPMAS) data in conjunction with “direct-polarization”, nonspinning CP, and T 1 measurements.
Bibliography:istex:E4E2B5451506137FA5778421E390131C7E4FCFB3
ark:/67375/TPS-6ZDG12KG-T
ISSN:0002-7863
1520-5126
DOI:10.1021/ja017711x