Angelica Sinensis Polysaccharide Prevents Hematopoietic Stem Cells Senescence in D-Galactose-Induced Aging Mouse Model

• Published in: Stem Cells International Vol. 2017; no. 2017; pp. 1 - 12-75
• Main Authors: Wang, Ya-Ping, Wang, Lu, Jing, Pengwei, Chen, Xiongbin, Xia, Jieyu, Li, Jing, Zhang, Yanyan, Mu, Xin-Yi, Song, Xiaoying
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Limiteds 01.01.2017; Hindawi Publishing Corporation; Hindawi; Hindawi Limited; Wiley
• Subjects: Aging; Aging (Biology); Angelica; Blood diseases; Bone marrow; Cell cycle; Chinese medicine; Deoxyribonucleic acid; DNA; DNA damage; Dong quai; Galactose; Gene expression; Hypotheses; Laboratory animals; Oxidative stress; Physiology; Polysaccharides; Regulation; Senescence; Signal transduction; Stem cells; Studies
• ISSN: 1687-966X; 1687-9678; 1687-9678
• DOI: 10.1155/2017/3508907

Age-related regression in hematopoietic stem/progenitor cells (HSC/HPCs) limits replenishment of the blood and immune system and hence contributes to hematopoietic diseases and declined immunity. In this study, we employed D-gal-induced aging mouse model and observed the antiaging effects of Angelica Sinensis Polysaccharide (ASP), a major active ingredient in dong quai (Chinese Angelica Sinensis), on the Sca-1+ HSC/HPCs in vivo. ASP treatment prevents HSC/HPCs senescence with decreased AGEs levels in the serum, reduced SA-β-Gal positive cells, and promoted CFU-Mix formation in the D-gal administrated mouse. We further found that multiple mechanisms were involved: (1) ASP treatment prevented oxidative damage as total antioxidant capacity was increased and levels of reactive oxygen species (ROS), 8-OHdG, and 4-HNE were declined, (2) ASP reduced the expression of γ-H2A.X which is a DNA double strand breaks (DSBs) marker and decreased the subsequent ectopic expressions of effectors in p16Ink4a-RB and p19Arf-p21Cip1/Waf senescent pathways, and (3) ASP inhibited the excessive activation of Wnt/β-catenin signaling in aged HSC/HPCs, as the expressions of β-catenin, phospho-GSK-3β, and TCF-4 were decreased, and the cyto-nuclear translocation of β-catenin was inhibited. Moreover, compared with the positive control of Vitamin E, ASP exhibited a better antiaging effect and a weaker antioxidation ability, suggesting a novel protective role of ASP in the hematopoietic system.