Life in the coffee-ring: how evaporation-driven density gradients dictate the outcome of inter-bacterial competition

• Main Authors: Yanni, David, Kalziqi, Arben, Thomas, Jacob, Ng, Siu Lung, Vivek, Skanda, Ratcliff, William C, Hammer, Brian K, Yunker, Peter J
• Format: Journal Article
• Language: English
• Published: 11.07.2017
• Subjects: Physics - Biological Physics; Physics - Soft Condensed Matter; Quantitative Biology - Cell Behavior
• DOI: 10.48550/arxiv.1707.03472

When a drop dries, it often leaves a ring-shaped stain through a ubiquitous phenomenon known as the coffee-ring effect. This also occurs when the liquid contains suspended microbes; evaporation leaves cells at higher concentrations in the ring than the drop interior. Using biofilm experiments and cellular automata simulations, we show that the physical structure created by the coffee-ring effect can dramatically alter the outcome of inter-bacterial competition. We experimentally study this effect using two strains of $\textit{Vibrio cholerae}$ that compete using a contact-dependent killing mechanism termed the type 6 secretion system. By creating a heterogeneous density profile, the coffee-ring effect changes the outcome of competition: the bacterial strain that wins in the low-density interior loses in the far denser ring. Through simulations parameterized with experimentally-determined density profiles, we recapitulate our experimental findings. We examine the role of a $\textit{V. cholerae}$ strain's frequency, its relative efficacy at killing competitors, and the initial concentration of cells in the droplet in determining the outcome of competition. By scaling from individual cellular interactions to overall changes in strain frequency, our work demonstrates how the coffee-ring effect plays a powerful role in structuring microbial communities, indirectly driving ecological changes in community composition.