Analysis and Confirmation of 1,3-DMAA and 1,4-DMAA in Geranium Plants Using High Performance Liquid Chromatography with Tandem Mass Spectrometry at ng/g Concentrations

• Published in: Analytical Chemistry Insights Vol. 2012; no. 7; pp. 59 - 78
• Main Authors: Fleming, Heather L., Ranaivo, Patricia L., Simone, Paul S.
• Format: Journal Article
• Language: English
• Published: London, England Libertas Academica 01.01.2012; SAGE Publishing; SAGE Publications; Sage Publications Ltd. (UK); Sage Publications Ltd
• Subjects: Dietary supplements; Dimethylamylamine; Liquid chromatography; Mass spectrometry; Original Research; Stereoisomers; geranium; natural product analysis; DMAA; mass spectrometry; HPLC
• ISSN: 1177-3901; 1177-3901
• DOI: 10.4137/ACI.S10445

1,3-Dimethylamylamine (1,3-DMAA) is a stimulant commercially sold in a variety of dietary supplements as a chemical species derived from geranium plants (Pelargonium graveolens). Whether 1,3-DMAA naturally occurs in geranium plants or other dietary ingredients, it has important regulatory and commercial ramifications. However, the analysis of 1,3-DMAA in geranium plants is not trivial due to low concentrations and a complex environmental matrix, requiring high selectivity and sensitivity. An extraction method combined with high performance liquid chromatography and tandem mass spectrometry is used to determine 1,3-DMAA and 1,4-dimethylamylamine (1,4-DMAA) concentrations in geranium plants with both external calibration and standard addition method. Samples from the Changzhou, Kunming, and Guiyang regions of China during both winter and summer were analyzed for 1,3-DMAA and 1,4-DMAA. The diastereomer ratios of the 1,3-DMAA stereoisomers of a racemic standard and the extracted plant were also quantified.