Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells

• Published in: ACS central science Vol. 7; no. 8; pp. 1419 - 1426
• Main Authors: Tyson, Jonathan, Hu, Kevin, Zheng, Shuai, Kidd, Phylicia, Dadina, Neville, Chu, Ling, Toomre, Derek, Bewersdorf, Joerg, Schepartz, Alanna
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 25.08.2021; American Chemical Society (ACS)
• Subjects: Absorption; Fluorophores; Heterocyclic compounds; INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Lasers; Quantum yield

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluor...