Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluor...

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Bibliographic Details
Published inACS central science Vol. 7; no. 8; pp. 1419 - 1426
Main Authors Tyson, Jonathan, Hu, Kevin, Zheng, Shuai, Kidd, Phylicia, Dadina, Neville, Chu, Ling, Toomre, Derek, Bewersdorf, Joerg, Schepartz, Alanna
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 25.08.2021
American Chemical Society (ACS)
Subjects
Absorption
Fluorophores
Heterocyclic compounds
INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Lasers
Quantum yield
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Summary:New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
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AC02-05CH11231
USDOE Office of Science (SC), Basic Energy Sciences (BES)
National Institutes of Health (NIH)
ISSN:2374-7943
2374-7951
DOI:10.1021/acscentsci.1c00670