Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluor...

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Published inACS central science Vol. 7; no. 8; pp. 1419 - 1426
Main Authors Tyson, Jonathan, Hu, Kevin, Zheng, Shuai, Kidd, Phylicia, Dadina, Neville, Chu, Ling, Toomre, Derek, Bewersdorf, Joerg, Schepartz, Alanna
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 25.08.2021
American Chemical Society (ACS)
Subjects
Absorption
Fluorophores
Heterocyclic compounds
INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Lasers
Quantum yield
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Abstract New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
AbstractList New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale , a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale 676sb , a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale 676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives. The bright and near-IR-emitting spontaneously blinking fluorophore Yale 676sb is paired with HMSiR to enable two-color live-cell single-molecule localization microscopy with no chemical additives and a single 642 nm laser.
Author Bewersdorf, Joerg
Chu, Ling
Zheng, Shuai
Hu, Kevin
Toomre, Derek
Schepartz, Alanna
Kidd, Phylicia
Dadina, Neville
Tyson, Jonathan
AuthorAffiliation Department of Chemistry
Department of Molecular and Cellular Biology
California Institute for Quantitative Biosciences (QB3)
Department of Molecular, Cellular, and Developmental Biology
Kavli Institute for Neuroscience
Nanobiology Institute
Department of Biomedical Engineering
Department of Cell Biology
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Snippet New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule...
New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule...
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SubjectTerms Absorption
Fluorophores
Heterocyclic compounds
INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Lasers
Quantum yield
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Title Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells
URI http://dx.doi.org/10.1021/acscentsci.1c00670
https://www.ncbi.nlm.nih.gov/pubmed/34471685
https://www.proquest.com/docview/2568597406
https://www.osti.gov/servlets/purl/2469690
https://pubmed.ncbi.nlm.nih.gov/PMC8393207
https://doaj.org/article/ad553efb5ff541d4b0fff037a0ffb785
Volume 7
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