Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis

• Published in: Journal of Traditional and Complementary Medicine Vol. 10; no. 6; pp. 529 - 543
• Main Authors: Chaudhary, Amrita, Bag, Swarnendu, Banerjee, Provas, Chatterjee, Jyotirmoy
• Format: Journal Article
• Language: English
• Published: Netherlands 國立臺灣大學食品與生物分子研究中心 01.11.2020; Elsevier Taiwan LLC; Elsevier
• Subjects: Angiogenesis; Diabetic wound; Jamun honey; Original; Reepithelialization; Wound closure; Jamun honey; Angiogenesis; Diabetic wound; Reepithelialization; Wound closure; DAB; EGF; IHC; H&E; VEGF; EMT; DBM; ECM; DMEM; VG; STZ; PI; HIF 1 α; MH; JH; JH, Jamun honey; STZ, Streptozotocin; VG, van Gieson’s; EMT, Epithelial–mesenchymal transition; EGF, Epidermal growth factor; DBM, Diabetic mice; HIF 1 α, Hypoxia-inducible factor 1 α; VEGF, Vascular endothelial growth factor; H&E, Hematoxylin and Eosin; MH, Manuka honey; DAB, 3,3′-Diaminobenzidine; ECM, Extracellular matrix; IHC, Immuno-histochemistry; PI, Povidine Iodine; DMEM, Dulbecco’s Modified Eagle Medium
• ISSN: 2225-4110; 2225-4110
• DOI: 10.1016/j.jtcme.2019.10.002

Diabetic patients are frequently afflicted with impaired wound healing where linear progression of molecular and cellular events compromised. Despite of meaningful progress in diabetic treatment, management of diabetic chronic wounds is still challenging. Jamun (Syzygium cumini) honey may be a promising candidate for diabetic wound healing and need to explore in detail. So present study was designed to evaluate the efficacy of Jamun honey (JH) for diabetic wound healing in in vitro wound (primary fibroblasts) model and in in vivo of diabetic mice (Streptozotocin induced) model. The fibroblast cell model was studied for migratory behaviour and myofibrolasts infiltration under honey interventions via scratch/migration assay, immuno-cytochemistry and western blot. We applied FDA approved Manuka honey (MH) as positive control and JH as test honey to evaluate wound re-epithelialization, sub-epithelial connective tissue modification and angiogenesis via histopathological and immuno-histochemical analysis. JH (0.1% v/v) dilution has notably improved wound closure, migration with concomitantα-SMA expressions in vitro. Topical application of JH in diabetic mice model showed significant (*p ≤ 0.05) wound closure, reepithelialization, collagen deposition (I/III) and balanced the myofibroblasts formation. It also modulated vital angiogenic markers (viz HIF-1α, VEGF, VEGF R-II) significantly (*p ≤ 0.05). All these observations depicted that JH promotes sequential stages of wound healing in diabetic mice model. The results of the present study established Jamun honey as good as Manuka honey considering wound closure, re-epithelialization, collagen deposition and pro-angiogenic potential.