Suppressive effects of co-stimulatory molecule expressions on mouse splenocytes by anti-allergic agents in vitro

• Published in: Mediators of Inflammation Vol. 2000; no. 2; pp. 69 - 75
• Main Authors: Ito, J, Asano, K, Tryka, E, Kanai, K, Yamamoto, S, Hisamitsu, T, Suzaki, H
• Format: Journal Article
• Language: English
• Published: United States Hindawi Limiteds 01.01.2000; Hindawi Limited
• Subjects: Animals; Anti-Allergic Agents - pharmacology; Antigens, CD - biosynthesis; B7-1 Antigen - biosynthesis; B7-2 Antigen; CD40 Antigens - biosynthesis; Cell Division; Cells, Cultured; Cromolyn Sodium - pharmacology; Dibenzazepines - pharmacology; Histamine H1 Antagonists - pharmacology; Imidazoles - pharmacology; Male; Membrane Glycoproteins - biosynthesis; Mice; Mice, Inbred BALB C; Spleen - cytology; Spleen - drug effects; Spleen - immunology; Anti-allergic agent; Inhibition; Spleen cell; Mouse; Expression; Co-stimulatory molecule
• ISSN: 0962-9351; 1466-1861
• DOI: 10.1080/096293500411523

THE influence of anti-allergic drugs, epinastine hydrochloride (EP) and disodium cromoglycate (DSCG), on the co-stimulatory molecule expression was examined using in vitro cell culture technique. Spleen cells obtained from BALB/c mice 10 days after immunization with haemocyanin absorbed to aluminium hydroxide were cultured in the presence of 100.0 μg /ml haemocyanin and various concentrations of the agents. Low concentrations (＜1.5x10^(-4) M) of EP and DSCG did not influence spleen cell blastic activity induced by antigenic stimulation, whereas these agents caused significant inhibition of spleen cell activation when 2x10^(-4) M of the agents were added to cell cultures . EP and DSCG also did not affect blastic activity of sensitized splenic T cells by anti-CD3 monoclonal antibody stimulation even when these cells were cultured in the presence of 2x10^(-4)M of the agents. We next examined the influence of EP and DSCG on the expression of co-stimulatory molecule s on spleen cells in response to antigenic stimulation. Sensitized spleen cells were cultured in the presence of 2 x10^(-4)M of the agents and the expression of molecules were examined by flow cytometer 24 h later. EP and DSCG suppressed the expression of costimulatory molecule, CD40 and CD80, but not CD86, on splenic B cells which were enhanced by antigenic stimulation in vitro.