Importance of Multidrug Efflux Pumps in the Antimicrobial Resistance Property of Clinical Multidrug-Resistant Isolates of Neisseria gonorrhoeae

• Published in: Antimicrobial agents and chemotherapy Vol. 58; no. 6; pp. 3556 - 3559
• Main Authors: Golparian, Daniel, Shafer, William M., Ohnishi, Makoto, Unemo, Magnus
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.06.2014
• Subjects: Anti-Bacterial Agents; Anti-Bacterial Agents - pharmacology; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacterial diseases; Bacterial diseases of the genital system; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Drug Resistance, Multiple, Bacterial - genetics; Epidemiology. Vaccinations; General aspects; Genes, MDR; Genes, MDR - physiology; Gonorrhea; Gonorrhea - microbiology; Human bacterial diseases; Humans; Infectious diseases; Mechanisms of Resistance; Medical sciences; Membrane Transport Proteins; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Microbial Sensitivity Tests; Mutation; Neisseria gonorrhoeae; Neisseria gonorrhoeae - drug effects; Neisseria gonorrhoeae - genetics; Pharmacology. Drug treatments; Human; Drug susceptibility test; Neisseriaceae; Antimicrobial agent; Infection; Sexually transmitted disease; Gonococcal infection; Neisseria gonorrhoeae; Membrane pump; Membrane transport; Multiple resistance; Bacteriosis; Bacteria; Micrococcales; Clinical isolate
• ISSN: 0066-4804; 1098-6596; 1098-6596
• DOI: 10.1128/AAC.00038-14

The contribution of drug efflux pumps in clinical isolates of Neisseria gonorrhoeae that express extensively drug-resistant or multidrug-resistant phenotypes has heretofore not been examined. Accordingly, we assessed the effect on antimicrobial resistance of loss of the three gonococcal efflux pumps associated with a known capacity to export antimicrobials (MtrC-MtrD-MtrE, MacA-MacB, and NorM) in such clinical isolates. We report that the MIC of several antimicrobials, including seven previously and currently recommended for treatment was significantly impacted.