Dynamics of Mycobacterium tuberculosis-Specific and Nonspecific Immune Responses in Women with Tuberculosis Infection during Pregnancy

• Published in: Microbiology spectrum Vol. 10; no. 5; p. e0117822
• Main Authors: Tesfaye, Fregenet, Sturegård, Erik, Walles, John, Bekele, Bayissa, Bobosha, Kidist, Björkman, Per, Jansson, Marianne
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 26.10.2022
• Subjects: Antigens, Bacterial; Chemokine CXCL10 - metabolism; Clinical Medicine; Clinical Microbiology; Cytokines; Female; HIV Infections; HIV-uninfected; Humans; Immunity; interferon-g inducible protein 10; Interferon-gamma; interferon-γ inducible protein 10; Interleukin 1 Receptor Antagonist Protein; Interleukin-2; Klinisk medicin; Latent Tuberculosis - diagnosis; Medical and Health Sciences; Medicin och hälsovetenskap; Mycobacterium tuberculosis; Mycobacterium tuberculosis antigen reactivity; Obstetrics, Gynecology and Reproductive Medicine; Pregnancy; Reproduktionsmedicin och gynekologi; Research Article; Transforming Growth Factor beta1; tuberculosis infection; Tuberculosis, Lymph Node; interferon-γ inducible protein 10; tuberculosis infection; Mycobacterium tuberculosis antigen reactivity; interleukin 2; pregnancy; transforming growth factor-beta1; HIV-uninfected
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.01178-22

The immune control of tuberculosis (TB) infection could be influenced by pregnancy. To elucidate this, we longitudinally characterized Mycobacterium tuberculosis (Mtb)-specific and nonspecific immune responses in women during pregnancy and postpartum. HIV-uninfected women without past or current active TB, and with blood samples available from the 1st/2nd trimester, 3rd trimester, and 9 months postpartum, were identified at Ethiopian antenatal care clinics. Twenty-two TB+ women and 10 TB- women, defined according to Mtb-stimulated interferon-γ levels (≥0.35 and <0.20 IU/mL, respectively, in the Quantiferon-TB Gold-Plus assay), were included in the study. Longitudinal dynamics of six cytokines (IL-1ra, IL-2, IP-10, MCP-2, MCP-3, and TGF-β1) were analyzed in supernatants from Mtb-stimulated and unstimulated whole blood. In TB+ women, Mtb-specific expression of IL-2 and IP-10 was higher at 3rd compared to 1st/2nd trimester (median 139 pg/mL versus 62 pg/mL,  = 0.006; 4,999 pg/mL versus 2,310 pg/mL,  = 0.031, respectively), whereas level of Mtb-triggered TGF-β1 was lower at 3rd compared to 1st/2nd trimester (-6.8 ng/mL versus 2.3 ng/mL,  = 0.020). Unstimulated IL-2, IP-10, and MCP-2 levels were increased postpartum, compared with those noted during pregnancy, in TB+ women. Additionally, postpartum levels of proinflammatory cytokines in unstimulated blood were higher in TB+ women, than in TB- women. None of the women developed active TB during follow-up. Taken together, dynamic changes of Mtb-specific cytokine expression revealed during the 3rd trimester in TB+ women indicate increased Mtb-antigen stimulation at later stages of pregnancy. This could reflect elevated bacterial activity, albeit without transition to active TB, during pregnancy. Tuberculosis (TB) is globally one of the most common causes of death, and a quarter of the world's population is estimated to have TB infection. The risk of active TB is increased in connection to pregnancy, a phenomenon that could be due to physiological immune changes. Here, we studied the effect of pregnancy on immune responses triggered in HIV-uninfected women with TB infection, by analyzing blood samples obtained longitudinally during pregnancy and after childbirth. We found that the dynamics of Mtb-specific and nonspecific immune responses changed during pregnancy, especially in later stages of pregnancy, although none of the women followed in this study developed active TB. This suggests that incipient TB, with elevated bacterial activity, occurs during pregnancy, but progression of infection appears to be counteracted by Mtb-specific immune responses. Thus, this study sheds light on immune control of TB during pregnancy, which could be of importance for future intervention strategies.