Single-Molecule Kinetics in Living Cells

In the past decades, advances in microscopy have made it possible to study the dynamics of individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be hidden in ensemble averages. More recently, single-molecule methods have been used to image, localize, and track ind...

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Published inAnnual review of biochemistry Vol. 88; no. 1; pp. 635 - 659
Main Authors Elf, Johan, Barkefors, Irmeli
Format Journal Article
LanguageEnglish
Published United States Annual Reviews 20.06.2019
Annual Reviews, Inc
Subjects
Biomolecules
Cells (biology)
Cytoplasm
fluorescence labeling
Kinetics
Macromolecules
simulated microscopy
single-molecule kinetics
single-molecule microscopy
single-molecule tracking
spot localization
single-molecule tracking
simulated microscopy
single-molecule microscopy
spot localization
fluorescence labeling
single-molecule kinetics
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Summary:In the past decades, advances in microscopy have made it possible to study the dynamics of individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be hidden in ensemble averages. More recently, single-molecule methods have been used to image, localize, and track individually labeled macromolecules in the cytoplasm of living cells, allowing investigations of intermolecular kinetics under physiologically relevant conditions. In this review, we illuminate the particular advantages of single-molecule techniques when studying kinetics in living cells and discuss solutions to specific challenges associated with these methods.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISBN:0824308883
9780824308889
ISSN:0066-4154
1545-4509
1545-4509
DOI:10.1146/annurev-biochem-013118-110801